設施農業：智慧感測【生物產業機電工程學系吳靖宙教授】

論文篇名 英文：An electrooxidative technique to fast fabricate copper phosphate electrodes capable of integrating high performance liquid chromatography for the label-free detection of fish freshness
中文：以電氧化法研製磷酸銅電極與高效液相層析法整合應用於免標定魚肉新鮮度檢測
期刊名稱 Food Chemistry
發表年份,卷數,起迄頁數 2018, 269, 16-23
作者 Yu-Han Hsieh, Ming-Yuan Lee, Ching-Chou Wu*(吳靖宙)
DOI 10.1016/j.foodchem.2018.06.125
中文摘要 本研究整合電氧化法研製磷酸銅(Cu3(PO4)2)電極於高效液相層析法(HPLC)應用於免標定魚肉新鮮度檢測。結果顯示在NaH2PO4 (pH 5.0)溶液中給予Epa1/2氧化電位10分鐘，可於銅箔電極表面製作出片狀的磷酸銅結構，且該磷酸銅複合物電極在20 mM NaH2PO4 pH 5.0-8.5溶液中，可對組織胺和組織酸具有良好的電催化活性。整合磷酸銅電極與流動注入分析(FIA)系統之檢測裝置對組織胺的線性範圍為2.5-250 ppm，檢測極限為0.15 ppm。而整合電極於HPLC在NaH2PO4 pH 7.5溶液檢測組織胺，其準確率與回收率分別為95.3%及101.1%。
英文摘要 simple and fast one-step electrooxidative method has been developed to monolithically produce a copper phosphate (Cu3(PO4)2) compound on a disposable copper tape, which can be integrated with high performance liquid chromatography (HPLC) for the estimation of fish freshness. The Cu3(PO4)2 compound of flake-like nanostructures was formed by applying a first anodic peak potential at the copper tape for 10 min in a 1M sodium dihydrogen phosphate (NaH2PO4) (pH 5.0) solution. The Cu3(PO4)2 electrodes can detect the oxidative reaction of histidine and histamine in 20mM NaH2PO4 solutions with pH 5.0–8.5. When integrating the electrodes with a flow injection system, the linear range and the calculated detection limit of histamine were respectively 2.5–250 ppm and 0.15 ppm. The electrodes integrated to HPLC can specifically detect the histamine concentrations in fish samples in the pH 7.5 NaH2PO4 solution, achieving an accuracy rate of 95.3% and a recovery rate of 101.1%.