生態農業：農業精準栽培管理技術開發【土壤環境科學系賴鴻裕副教授】

論文篇名 英文：Effect of inoculation with arbuscular mycorrhizal fungi and blanching on the bioaccessibility of heavy metals in water spinach (Ipomoea aquatica Forsk.)
中文：接種菌根真菌及川燙對蕹菜累積重金屬生物可及性之影響
期刊名稱 Ecotox. Environ. Safe.
發表年份,卷數,起迄頁數 2018, 162, 563-570
作者 Lam, C.M., and H.Y. Lai*(賴鴻裕)
DOI 10.1016/j.ecoenv.2018.07.047
中文摘要 植物對重金屬的耐受及其解毒機制與其次細胞的分布及化學型態有關，本研究將蕹菜種植在受單一或多種重金屬污染的土壤中，並利用接種菌根菌增加蕹菜對於磷的累積，希望藉此降低重金屬在蕹菜可食用部位的累積濃度，並探討川燙及模擬消化對重金屬生物可及性之影響。試驗結果顯示，本研究所使用的蕹菜對重金屬具有高累積性，接種菌根菌可以促進蕹菜之生長並降低對鎳的累積，但對鎘則否。可溶及無機型態分別是鎘及鎳主要存在的次細胞及化學型態，而此兩種重金屬的移動性也比鉻高。與未川燙之蕹菜相比，川燙可以讓鎘、鉻及鎳降低45–84%，而約有32–55%、16–50%及27–40%的鎘、鉻及鎳具生物可及性，可以被消化系統所吸收。
英文摘要 A plant's tolerance to heavy metals (HMs) and its detoxification mechanisms are associated with the subcellular distribution of HMs and their chemical forms. In this study, water spinach (Ipomoea aquatica Forsk.) was grown in two soils contaminated with a single HM (cadmium, Cd) or combined HMs (Cd and nickel, Ni). Inoculation of arbuscular mycorrizal fungi (AMF) was conducted to increase the accumulation of phosphorus (P) in plants. One major exception was to decrease the migration and accumulation of HMs in edible parts by the formation of P-HM complexes. The effects of blanching and simulated digestion on bioaccessibility were also assessed. The experimental results showed that the water spinach species used in this study had a high capacity to accumulate HMs. AMF treatment improved water spinach growth and decreased the accumulation of Ni but not that of Cd. Soluble and inorganic Cd and Ni were the major subcellular fractions and chemical forms in water spinach; these two HMs also exhibited higher migration capacities in comparison to chromium (Cr). Relative to raw tissues, 45–84% of Cd, Cr, and Ni were leached after blanching. Approximately 32–55%, 16–50%, and 27–40% of Cd, Cr, and Ni, respectively, were bioaccessible and could be metabolized by in vitro digestive fluids.