2021 Focus:
Nowadays, agricultural waste is the organic waste with the highest proportion. Therefore, we used Cellulosome to decompose agricultural waste to produce liquid of  saccharification, then apply to the production of biostimulant (PQQ) and return to agricultural use as our aim. We uses novel synthetic biology technology to breakthrough the limitation of the number of genes that can be transplanted by molecular biotechnology in the past. Moreover, Bacillus subtilis containing Cellulosome gene group and PQQ-related gene group has been constructed. Cellulosome is used to hydrolyze agricultural waste and generate liquid of  saccharification. After that, mixed with nutrient substances such as okara and trace elements as a medium for PQQ productive Bacillus. PQQ productive Bacillus simulate the symbiosis of endophytes that promote growth rate of plants. As result, we demonstrate how to transfer agricultural waste to higher economic product. Moreover, pour bacteria containing PQQ could benefit plants growth, such as rice, lettuce, melon, chili, etc.


2022 Focus:
Based on the result, saccharification process by cellulosome, we’ll develop purification process to enhance economic value of agricultural waste, and investigate the signaling pathway of plant that effect by PQQ in molecular level. And we’ll try to industrialize our results, reduce process costs and assess the feasibility of commercialization, to achieve Circular agriculture.