新穎材料農業：農業新穎材料在植物保健開發、應用與機理【植物病理學系/鍾文鑫教授】
| 論文篇名 | 英文：Antifungal susceptibility of the clinical and environmental strains of Cryptococcus gattii sensu lato in Taiwan 中文：臺灣臨床與環境Cryptococcus gattii sensu lato對藥劑感受性 |
| 期刊名稱 | MYCOSES |
| 發表年份,卷數,起迄頁數 | 2022 |
| 作者 | Lin, Kuo-Hsi; Lai, Yi-Chyi; Lin, Yi-Pei; Ho, Mao-Wang; Chen, Yee-Chun; Chung, Wen-Hsin(鍾文鑫)* |
| DOI | 10.1111/myc.13520 |
| 中文摘要 | 由 Cryptococcus gattii sensu lato 引起的人類隱球菌病的罕見發生，導致難以確定這種潛在致命病原之間對抗藥性現況，而這研究可能對治療隱球菌病至關重要。所以本研究目的為確認臺灣不同來源C. gattii s.l.對藥劑的感受性。本研究供試的菌株包含，來自環境的104株C. gattii s.l.菌株(ST7、ST106、ST274、ST328、ST546、ST548及ST630分子型)及來自臨床的21株菌株(ST7、ST44、ST06、ST274、ST328及ST329分子型)。透過最小抑制濃度(MIC)測試，分析來自環境與臨床C. gattii s.l.菌株對6種殺菌劑(itraconazole、fluconazole、voriconazole、posaconazole、flucytosine及amphotericin B)的抗感性。MIC測試結果指出，來自環境的C. gattii s.l.菌株對azoles與flucytosine的感受性高，且各分子型之間具有顯著差異。此外，來自臺灣各位之C. gattii s.l.菌株對azoles藥劑的感受性會因區域性不同而有差異。本研究結果證實，透過對供試藥劑感受性無法區分來自臨床或環境的C. gattii s.l.菌株。此外，建立以 ST 為導向的國內抗藥性數據庫，有助於治療 C. gattii s.l. 所引起的隱球菌病。 |
| 英文摘要 | Background: The rare occurrence of human cryptococcosis caused by Cryptococcus gattii sensu lato leads to difficulties in establishing the antifungal susceptibility profile between species of this potentially lethal pathogen, which may be crucial for treating cryptococcosis. Objective: To establish an antifungal susceptibility profile of C. gattii s.l. in Taiwan. Methods: A total of 104 environmental C. gattii s.l. strains (including multilocal sequence typing ST7, ST106, ST274, ST328, ST546, ST548 and ST630) and 21 previously collected clinical strains (including ST7, ST44, ST06, ST274, ST328 and ST329) were included in this study. We determined the minimum inhibitory concentrations (MICs) of six antifungal agents (itraconazole, fluconazole, voriconazole, posaconazole, flucytosine and amphotericin B) against environmental C. gattii s.l. strains and compared the antifungal susceptibility profiles of environmental strains with those of clinical strains. Results: The antifungal susceptibility data demonstrated that the MICs of antifungal agents against environmental strains were comparable to those against clinical strains. Compared with strains of Cryptococcus deuterogattii, those of C. gattii sensu stricto were more susceptible to azoles and flucytosine. The differences in antifungal susceptibility between the strains of each sequence type (ST) were significant. Correlation analysis of MICs revealed cross-resistance between azoles in environmental strains of C. gattii s.l. Geographic differences in the antifungal susceptibility of C. gattii s.l. isolated from different cities in Taiwan were observed in this study. Conclusion: Clinical and environmental strains were indistinguishable in antifungal susceptibility. The antifungal susceptibility of C. gattii s.l. is associated with STs. Therefore, establishing an ST-oriented domestic antifungal susceptibility database may help treat C. gattii s.l.-induced cryptococcosis. |
| 發表成果與本中心研究主題相關性 | 隱球菌(Cryptococcus spp.)為農業重要微生物，普遍存在於農業環境中，該屬微生物屬擔子酵母菌，可做為生物防治資材，用於防治作物貯藏病害。於隱球菌屬中的格特隱球菌(C. gattii)為人體伺機性病原菌，由於該類菌株具有污染作物，進而感染人類的風險。由於隱球菌普存在於農業環境，易接觸農業化學藥劑，因此釐清環境與臨床之C. gattii菌株對不同化學藥劑之抗感性非常重要，與本中心的農業永續有相關性。 |
