設施農業：畜牧廢棄物於小球藻自動栽培與禽類再利用【生物產業機電工程學系/吳靖宙教授】
| 論文篇名 | 英文：A microfluidic chip integrating electrochemical impedimetric immunosensors constructed by top-bottom opposite electrodes for rapid detection of peanut allergen-Ara h 1 中文：整合上下相對電極之電化學阻抗免疫感測器的微流體晶片於花生過敏原-Ara h 1的快速檢測 |
| 期刊名稱 | Sensors and Actuators: B. Chemical |
| 發表年份,卷數,起迄頁數 | 2023, 396, 134637 |
| 作者 | Wu, Ching-Chou(吳靖宙)*; Singh, Kanishk; Ye, Jin-Xin; Chuang, Yu-Sheng; Wen, Hsiao-Wei |
| DOI | 10.1016/j.snb.2023.134637 |
| 中文摘要 | 開發快速、超靈敏的免疫感測器對於檢測食品中的過敏原在食品安全領域有重要意義。本究提出一種由上下相對的電極組成的微流體生物感測裝置，用於建構花生過敏原-Ara h 1免疫感測器晶片和微流體裝置。在最佳流體操作(OFM)條件下，以50 μL/min流率輸送80 μL的含有2.5 mM Fe(CN)63−/4− 與Ara h 1 的磷酸鹽緩衝溶液作用2.5 min，可得較好的免疫反應。透過電化學阻抗分析免標記免疫感測器的電子轉移電阻（Ret）來量化感測晶片的Ara h 1 免疫反應。在執行一次 OFM 後，線性範圍和檢測極限分別為 1 pg/mL−10 ng/mL 和 3.9 fg/mL。此外，Ret 響應隨著OFM 次數的增加而增加。此感測裝置可以快速檢測Ara h 1 濃度。從標準花生醬中萃取的稀釋上清液的回收率可達95-103%。此微流體裝置整合電化學阻抗的免疫感測器可呈現出快速和超靈敏的感測特性，具有極佳潛力實現食品中過敏原的免標籤檢測。 |
| 英文摘要 | Developing a rapid and ultrasensitive immunosensor is significant for detecting allergenic contamination in food safety fields. This study reports a microfluidic biosensing device consisting of two top-bottom opposite electrode chips and a channel slab for constructing peanut allergen-Ara h 1 immunosensors. Under optimal fluidic manipulation (OFM), transporting an 80 μL aliquot of Ara h 1-containing phosphate buffer solution spiked by 2.5 mM Fe(CN)63−/4− mediator with a 50 μL/min flow rate for 2.5 min, the device can obtain better immunoreaction. The electron transfer resistance (Ret) of the label-free immunosensors was analyzed by electrochemical imped-ance spectroscopy (EIS) to quantify Ara h 1 immunoreaction. After performing OFM once, the linear range and the limit of detection were 1 pg/mL− 10 ng/mL and 3.9 fg/mL, respectively. Furthermore, the Ret response of the immunosensors increased with OFM times. The sensing device can detect the Ara h 1 concentration with good recovery (95 −103%) for the diluted supernatant extracted from standard peanut butter. The microfluidic device integrating EIS-based immunosensors exhibits rapid and ultrasensitive sensing properties with promising po-tential for label-free detection of allergens in food products. |
| 發表成果與本中心研究主題相關性 | 該論文把電化學阻抗免疫感測晶片整合到微流體系統中可在2.5 min內進行食品中過敏原的快速檢測，且其靈敏度可低達3.9 fg/mL，這對食品加工中可能會產生的食安問題可進行把關，該系統亦可應用於汙染源分子、細菌、動物健康指標蛋白的快速檢測。 |
