設施農業：智慧感測【生物產業機電工程學系吳靖宙教授】

論文篇名 英文：A disposable non-enzymatic histamine sensor based on the nafioncoated copper phosphate electrodes for estimation of fish freshness
中文：以nafion塗佈磷酸銅電極之可拋式非酵素型組織胺感測器於魚肉新鮮度測定
期刊名稱 Electrochimica Acta
發表年份,卷數,起迄頁數 2018, 283, 772-779
作者 Ming-Yuan Lee, Ching-Chou Wu*(吳靖宙), Megawati Intan Sari, Yu-Han Hsieh
DOI 10.1016/j.electacta.2018.05.148
中文摘要 組織胺是一重要指標在估算過敏反應和食物新鮮度。本研究是發展一單一步驟浸泡和塗佈方式以快速產生電化學組織胺感測器之nafion塗佈磷酸銅(copper phosphate, Cu3(PO4)2)電極。以銅電沉積之網版印刷碳電極(screen printed carbon electrodes, SPCEs)浸泡20分鐘後，可以快速形成薄片狀Cu3(PO4)2奈米結構。此外，1%的nafion塗佈於Cu3(PO4)2/SPCEs在pH 8.5溶液中檢測組胺具有良好的選擇性。此nafion/Cu3(PO4)2/SPCEs可以整合於流動式注入系統量測組織胺，在5500 ppm範圍內具有良好相關係數(R = 0.999)，檢測極限為3.0 ppm。此外，由nafion/Cu3(PO4)2/SPCEs檢測腐爛45小時之魚樣本組織胺濃度與商業化液相質譜層析法測得的值偏差小於10%。此nafion/Cu3(PO4)2/SPCEs具有建立魚肉新鮮度而無須昂貴分離裝置及標定過程。
英文摘要 Histamine is an important index for the estimation of allergic reaction and food freshness. This study develops a single-step dipping and coating method to fast produce an electrochemical histamine sensor based on a nafion-coated copper phosphate (Cu3(PO4)2)-dominated electrode. The flake-like Cu3(PO4)2 nanostructures can be fast formed on Cu-electrodeposited screen printed carbon electrodes (SPCEs) within 20 min. Moreover, the 1% nafion-coated Cu3(PO4)2/SPCEs presented good selectivity for the detection of histamine in the pH 8.5 solution. The nafion/Cu3(PO4)2/SPCEs can be integrated with a flowinjection device for the detection of histamine with a good correlation coefficient (R ¼ 0.999) in the range of 5e500 ppm. The calculated limit of detection was 3.0 ppm. Moreover, the histamine concentration of 45 h-rotten fish samples detected by the nafion/Cu3(PO4)2/SPCEs had a less than 10% deviation from that measured by the commercialized liquid chromatography-mass spectroscopy. The nafion/Cu3(PO4)2/ SPCEs have great promise for estimating the freshness of fish meat without expensive separation equipment and labor-intensive labeling procedures.