循環農業：農業有機循環再利用的多合一創新碳匯技術開發【土壤環境科學系/沈佛亭教授】
| 論文篇名 | 英文：Comprehensive insights into the impact of bacterial indole-3-acetic acid on sensory preferences in Drosophila melanogaster 中文：細菌產生之吲哚乙酸對於果蠅感知能力之影響研究 |
| 期刊名稱 | Scientific reports |
| 發表年份,卷數,起迄頁數 | 2024; 14:8311 |
| 作者 | Aziz, Raifa Abdul; Ramesh, Poornima; Suchithra, Kokkarambath Vannadil; Stothard, Paul; Narayana, Vanya Kadla; Raghu, Shamprasad Varija; Shen, Fo-Ting(沈佛亭)*; Young, Chiu-Chung(楊秋忠); Prasad, T. S. Keshava; Hameed, Asif |
| DOI | 10.1038/s41598-024-58829-7 |
| 中文摘要 | 許多自環境或臨床檢體中分離的細菌具有產生吲哚乙酸的能力，此一衍生自色胺酸之訊號分子可作為調節植物生長與發育賀爾蒙，但對於非植物類之真核生物影響則少有研究。本研究旨在探討一株可利用酒精作為碳源生長並產生吲哚乙酸之Pseudomonas juntendi，對於果蠅Drosophila melanogaster氣味辨識、運動性與存活能力之影響。利用高解析質譜分析經餵食吲哚乙酸後之果蠅蛋白質體，發現與腦部神經、聽覺與視覺感知、能量代謝相關之蛋白質表現量明顯提高，顯示細菌產生之吲哚乙酸對果蠅腸-腦軸線失衡具潛在影響。 |
| 英文摘要 | Several bacteria of environmental and clinical origins, including some human-associated strains secrete a cross-kingdom signaling molecule indole-3-acetic acid (IAA). IAA is a tryptophan (trp) derivative mainly known for regulating plant growth and development as a hormone. However, the nutritional sources that boost IAA secretion in bacteria and the impact of secreted IAA on non-plant eukaryotic hosts remained less explored. Here, we demonstrate significant trp-dependent IAA production in Pseudomonas juntendi NEEL19 when provided with ethanol as a carbon source in liquid cultures. IAA was further characterized to modulate the odor discrimination, motility and survivability in Drosophila melanogaster. A detailed analysis of IAA-fed fly brain proteome using high-resolution mass spectrometry showed significant (fold change, ± 2; p ≤ 0.05) alteration in the proteins governing neuromuscular features, audio-visual perception and energy metabolism as compared to IAA-unfed controls. Sex-wise variations in differentially regulated proteins were witnessed despite having similar visible changes in chemo perception and psychomotor responses in IAA-fed flies. This study not only revealed ethanol-specific enhancement in trp-dependent IAA production in P. juntendi, but also showed marked behavioral alterations in flies for which variations in an array of proteins governing odor discrimination, psychomotor responses, and energy metabolism are held responsible. Our study provided novel insights into disruptive attributes of bacterial IAA that can potentially influence the eukaryotic gut-brain axis having broad environmental and clinical implications. |
| 發表成果與本中心研究主題相關性 | 本研究旨在探討具有生成吲哚乙酸能力之細菌，除可調節植物生長與發育外，對於果蠅腦部神經發育、聽覺與視覺感知、能量代謝之影響，研究成果有助於探討農業永續管理下之土壤細菌與果蠅交互作用。 |
