生態農業：土壤碳蓄存之預測模型優化及驗證【土壤環境科學系/鄒裕民特聘教授】
| 論文篇名 | 英文：Design and assessment of highly stable norfloxacin/ Ca-montmorillonite (STx-1b) as oral beads 中文：高穩定性諾氟沙星/鈣蒙脫石 (STx-1b) 口服微珠的設計與評估 |
| 期刊名稱 | Applied Clay Science |
| 發表年份,卷數,起迄頁數 | 2024, 258, no.107508 |
| 作者 | Chang, Po-Hsiang; Liang, Chenxing; Yang, Yating; Wen, Yaqi; Lee, Cheng-, I; Ho, Juin-Yen; Chen, Chien-Yen; Tzou, Yu-Min(鄒裕民)* |
| DOI | 10.1016/j.clay.2024.107508 |
| 中文摘要 | 這項研究利用了海藻酸鈉（SA），該材料因其能夠選擇性地與某些二價陽離子交聯形成離子凝膠而聞名，使其成為凝膠材料的理想載體。在本研究中，我們採用無毒的鈣蒙脫石（STx-1b）作為基礎材料，並與SA混合生產含有諾氟沙星（NF）的複合微珠，用作口服微珠。在評估了市面上可用的 NF 濃度後，我們選擇了最低濃度 115 mg g−1 進行抗菌功效測試，並進行了一系列批量研究。在這些實驗之前，我們確認複合珠在 8 小時的加載期間成功封裝了 NF，並優化了各種參數，包括珠穩定性、NF 加載量和時間以及解吸速率。值得注意的是，只有 10% 的藥物在酸性條件（pH 2，模擬胃液）下解吸，顯示在到達腸道之前 NF 的損失最小，珠子沒有崩解。此複合珠對大腸桿菌（E. coli）表現出顯著的抗菌功效，凸顯了它們作為口服藥物輸送領域競爭替代品的潛力。此外，利用掃描電子顯微鏡（SEM）、X射線衍射（XRD）和熱重分析（TGA）來表徵複合珠。對於過期珠子的處理，TGA 結果表明，將珠子加熱至 350 °C 將有利於其分解，從而最大限度地減少對環境的影響。因此，由SA和STx-1b組成的複合微珠適合用作口服微珠。 |
| 英文摘要 | This study utilized sodium alginate (SA), known for its ability to selectively cross-link with certain divalent cations to form ionic gels, making it an ideal carrier for gel materials. In this study, we employed non-toxic Ca-montmorillonite (STx-1b) as the foundational material and mixed it with SA to produce composite beads containing norfloxacin (NF) for use as oral beads. After evaluating the available concentrations of NF in the market, we selected the lowest concentration of 115 mg g−1 for our antibacterial efficacy tests, which were conducted as a series of batch studies. Before these experiments, we confirmed that the composite beads successfully encapsulated NF during an 8-h loading period and optimized various parameters including bead stability, the amount and time of NF loading, and desorption rates. Notably, only 10% of the drug was desorbed under acidic conditions (pH 2, simulating gastric juice), indicating minimal loss of NF before reaching the intestinal tract, with the beads exhibiting no disintegration. The composite beads demonstrated significant antibacterial efficacy against Escherichia coli (E. coli), highlighting their potential as competitive alternatives in the field of oral drug delivery. Furthermore, scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) were utilized to characterize the composite beads. For the disposal of expired beads, TGA results indicated that heating the beads to 350 °C would facilitate their disintegration, thereby minimizing environmental impact. Consequently, the composite beads composed of SA and STx-1b are suitable for use as oral beads. |
| 發表成果與本中心研究主題相關性 | 黏土礦物為天然無毒的材料, 它不僅可以保護有機分子不被微生物利用降解, 更可以與其它天然物結合來作為口服藥物的carrier, 本研究即將含鈣的黏土與海藻酸鈉混和生成複合材料, 使其可以將目標藥物諾氟沙星在此複合物的保護下(耐酸及耐高溫), 送至受致病細菌感染的部位, 達成抗菌的目的, 本研究可將土壤的主成分應用於醫學領域, 擴大研究中心在研究領域的廣度。 |
