Recycling Agriculture: Development of Integrated All-in-One Technology for the Innovative carbon sequestration of Organic Circular Agriculture【Department of Soil Environmental Sciences / Young, Chiu-Chung / Academician】
循環農業：農業有機循環再利用的多合一創新碳匯技術開發【土壤環境科學系/楊秋忠院士】
論文篇名 英文：Genomic potential for inorganic carbon sequestration and xenobiotic degradation in marine bacterium Youngimonas vesicularis CC-AMW-ET affiliated to family Paracoccaceae
中文：副球菌科海洋細菌 Youngimonas vesillaryis CC-AMW-ET 中無機碳封存和外源物質降解的基因組證據
期刊名稱 ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
發表年份,卷數,起迄頁數 2023; DOI: 10.1007/s10482-023-01881-6
作者 Hameed, Asif*; Suchithra, Kokkarambath Vannadil; Lin, Shih-Yao; Stothard, Paul; Young, Chiu-Chung(楊秋忠)*
DOI 10.1007/s10482-023-01881-6
英文摘要 Ecological studies on marine microbial communities largely focus on fundamental biogeochemical processes or the most abundant constituents, while minor biological fractions are frequently neglected. Youngimonas vesicularis CC-AMW-ET, isolated from coastal surface seawater in Taiwan, is an under-represented marine Paracoccaceae (earlier Rhodobacteraceae) member. The CC-AMW-ET genome was sequenced to gain deeper insights into its role in marine carbon and sulfur cycles. The draft genome (3.7 Mb) contained 63.6% GC, 3773 coding sequences and 51 RNAs, and displayed maximum relatedness (79.06%) to Thalassobius litoralis KU5D5T, a Roseobacteraceae member. While phototrophic genes were absent, genes encoding two distinct subunits of carbon monoxide dehydrogenases (CoxL, BMS/Form II and a novel form III; CoxM and CoxS), and proteins involved in HCO3- uptake and interconversion, and anaplerotic HCO3- fixation were found. In addition, a gene coding for ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, form II), which fixes atmospheric CO2 was found in CC-AMW-ET. Genes for complete assimilatory sulfate reduction, sulfide oxidation (sulfide:quinone oxidoreductase, SqrA type) and dimethylsulfoniopropionate (DMSP) cleavage (DMSP lyase, DddL) were also identified. Furthermore, genes that degrade aromatic hydrocarbons such as quinate, salicylate, salicylate ester, p-hydroxybenzoate, catechol, gentisate, homogentisate, protocatechuate, 4-hydroxyphenylacetic acid, N-heterocyclic aromatic compounds and aromatic amines were present. Thus, Youngimonas vesicularis CC-AMW-ET is a potential chemolithoautotroph equipped with genetic machinery for the metabolism of aromatics, and predicted to play crucial roles in the biogeochemical cycling of marine carbon and sulfur.
發表成果與本中心研究主題相關性 Youngimonas vesillaryis CC-AMW-ET 是從台灣沿海表層海水中分離出來的，是一種代表性不足的海洋副球菌科（早期紅桿菌科）成員。發現了編碼一氧化碳脫氫酶的兩個不同亞基（CoxL、BMS/II 型和新型III 型；CoxM 和CoxS）的基因，以及參與HCO3- 攝取和相互轉化以及回補充HCO3- 固定的蛋白質。此外，在 CC-AMW-ET 中也發現了編碼核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO，II 型）的基因，該基因可固定大氣中的二氧化碳。 也鑑定了完全同化硫酸鹽還原、硫化物氧化（硫化物：醌氧化還原酶，SqrA 型）和二甲基磺酰基丙酸（DMSP）裂解（DMSP 裂合酶，DddL）的基因。 此外，還有降解芳香族碳氫化合物如奎寧酸、水楊酸、水楊酸酯、對羥基苯甲酸酯、兒茶酚、龍膽酸、黑黑酸、原兒茶酸、4-羥基苯乙酸、N-雜環芳香族化合物和芳香胺的基因。 因此，Youngimonas vesillaryis CC-AMW-ET是一種潛在的化學自營生物，配備有芳香族化合物代謝的遺傳機制，預計將在海洋碳和硫的生物地球化學循環中發揮關鍵作用。