新穎材料農業：農業新穎材料在植物保健開發、應用與機理【植物病理學系/鍾文鑫教授】
| 論文篇名 | 英文：Molecular epidemiology and phylogenetic analyses of environmental and clinical isolates of Cryptococcus gattii sensu lato in Taiwan 中文：來自臺灣環境與臨床之格特隱球菌(Cryptococcus gattii)的分子流行病學與親緣性分析 |
| 期刊名稱 | Mycoses |
| 發表年份,卷數,起迄頁數 | 2021, 64, 324-335 |
| 作者 | Lin, Kuo-Hsi; Lin, Yi-Pei; Ho, Mao-Wang; Chen, Yee-Chun; Chung, Wen-Hsin(鍾文鑫)* |
| DOI | 10.1111/myc.13195 |
| 中文摘要 | 由於格特隱球菌(Cryptococcus gattii)引起的病害較少，導致研究該病原之分子流行病學的困難度。本研究為建立臺灣格特隱球菌之分子流行族群多型性，並了解本土菌株與全球菌株之基因相關性，因此自環境與臨床廣泛蒐集C. gattii菌株進行相關研究。相關研究透過多位點序列分型(Multilocus sequence typing, MLST)方式，針對來自臺灣環境與臨床菌株進行多序列增幅、定序及分析。結果得知，於環境中C. gattii之分離率為16.7% (104/622)，所得104株C. gattii菌株，其MLST分成七種類型，而環境菌株與臨床菌株之間存在有基因與地理相關係性。另與全球C. gattii菌株分析結果，顯示臺灣C. gattii菌系和南美及南亞的菌系較接近。另透過菌株再重組測試，證實臺灣的C. gattii菌系具有無性或透有性過程進行繁殖，而C. deuterogattii是透過無性繁殖。 |
| 英文摘要 | The rare occurrence of cryptococcosis caused by Cryptococcus gattii sensu lato (C. gattii s.l.) leads to the difficulties in studying the molecular epidemiology of this globally emerging disease. To establish the molecular epidemiological profile of C. gattii s.l. in Taiwan, and understand the genetic relationship between locally endemic and global isolates. A nationwide survey on environmental C. gattii s.l. in Taiwan was conducted from 2017 to 2019. The geographic distribution and molecular epidemiology based on multilocus sequence typing (MLST) data of the environmental isolates were compared with 18 previously collected clinical isolates. Phylogenetic analysis was performed to elucidate the genetic relationship between the global isolates and the isolates endemic to Taiwan. From a total of 622 environmental samples, 104 (16.7%) were positive for C. gattii s.l.. Seven sequence types were identified among the environmental isolates. The genetic population structure showed that the environmental and clinical isolates were closely linked by sequence types and geographical locations. Phylogenetic analysis revealed the association between the C. gattii s.l. isolates in Taiwan and those from South America and South Asia. The recombination test suggested that, in Taiwan, the C. gattii sensu stricto (C. gattii s.s). isolates undergo clonal reproduction and sexual recombination, whereas C. deuterogattii isolates were clonal. |
| 發表成果與本中心研究主題相關性 | 隱球菌(Cryptococcus spp.)為農業重要微生物，普遍存在於農業環境中，該屬微生物屬擔子酵母菌，可做為生物防治資材，用於防治作物貯藏病害。於隱球菌屬中的格特隱球菌(C. gattii)為人體伺機性病原菌，由於該類菌株具有污染作物，進而感染人類的風險，因此釐清環境與臨床之C. gattii菌株間的關係有其重要性，與本中心的農業永續有相關性。 |
