循環農業：農業有機循環再利用的多合一創新碳匯技術開發【土壤環境科學系/楊秋忠院士】
| 論文篇名 | 英文：Neopusillimonas aromaticivorans sp. nov. isolated from poultry manure 中文：一株分離自禽畜糞可分解環狀芳香族碳氫化合物之台灣本土新穎細菌 |
| 期刊名稱 | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY |
| 發表年份,卷數,起迄頁數 | 2023, 73(11) |
| 作者 | Lin, Shih-Yao; Lin, Tzu-Yu; Hameed, Asif; Tang, Yu-Shan; Young, Chiu-Chung(楊秋忠)* |
| DOI | 10.1099/ijsem.0.006146 |
| 英文摘要 | A polyphasic taxonomic approach was used to characterize a novel bacterium, designated strain CC-YST667T, isolated from poultry manure sampled in Taiwan. The cells were observed to be aerobic, motile, and non-spore-forming rods, displaying positive reactions for oxidase. Optimal growth of strain CC-YST667T was observed at 25 °C, pH 8.0, and with 1 % (w/v) NaCl. The polar lipid profile was consisted of phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and multiple unidentified polar lipids. The major polyamine was spermidine. The major cellular fatty acids (>5 %) included C16:0, C17:0 cyclo, C19:0 cyclo ω8c, and C14:0 3OH / iso-C16:1 I. Based on analysis of 16S rRNA gene sequences, this isolate showed the closest phylogenetic relationship with ‘Neopusillimonas minor’ (with 98.2 % similarity) and Paralcaligenes ureilyticus (with 97.3 % similarity) of the Alcaligenaceae family. The draft genome (3.3 Mb) with a DNA G+C content of 57.2 mol%, and harbored various genes involved in the biodegradation of aromatic hydrocarbons. Strain CC-YST667T shared highest orthologous average nucleotide identity (OrthoANI) with the type strains of the genera Neopusillimonas (72.4‒77.9 %, n=2), Pusillimonas (72.8‒73.0 %, n=2), and Pollutimonas (71.7‒73.0 %, n=5). Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, OrthoANI, digital DDH, and the phylogenomic placement, strain CC-YST667T is considered to represent a novel Neopusillimonas species, for which the name Neopusillimonas aromaticivorans sp. nov. is proposed. The type strain is CC-YST667T (=BCRC 81321T =JCM 34761T). |
| 發表成果與本中心研究主題相關性 | 本研究的目的是描述一株從台灣禽畜糞樣品中分離出可可分解環狀芳香族碳氫化合物之台灣本土新穎細菌，並使用多相分類方法進行鑑定。觀察到細胞是需氧的、能動的、不形成孢子的桿狀細胞，顯示氧化酶的陽性反應。在 25 °C、pH 8.0 和 1 % (w/v) NaCl 下觀察到菌株 CC-YST667T 的最佳生長。極性脂質譜由磷脂醯單甲基乙醇胺、磷脂醯甘油、二磷脂醯甘油、磷脂醯乙醇胺和多種未鑑定的脂質極性組成。根據 16S rRNA 基因序列分析，基因組（3.3 Mb）的 DNA G+C 含量為 57.2 mol%，並包含與芳香烴生物降解有關的各種基因。菌株CC-YST667T與新微單胞菌屬(72.4-77.9 %, n=2)、微單胞菌屬(72.8-73.0 %, n=2) 及污染單胞菌屬(71.7-73.0) 模式菌株具有最高的直系同源平均核苷酸同一性。基於其獨特的系統發育、表型和化學分類學特徵以及比較16S rRNA 基因序列、OrthoANI、dDDH 和系統發育位置的結果，菌株CC-YST667T 被認為代表了Neopusillimonas菌屬中的新菌種，其名稱命名為Neopusillimonas aromaticivorans。 |
