新穎材料農業：農業新穎材料在農產品保鮮開發、應用與機理【土壤環境科學系/林耀東特聘教授】
| 論文篇名 | 英文：A solution of identifying biophysical properties and 3D cellular structure of visible-light-driven photocatalytic inactivated Staphylococcus aureus 中文：一種識別可見光驅動光催化滅活金黃色葡萄球菌生物物理特性和3D細胞結構之測定方法 |
| 期刊名稱 | Chemical Engineering Journal |
| 發表年份,卷數,起迄頁數 | 2021, 421, 127880 |
| 作者 | Tzeng, Jing-Hua; Weng, Chih-Huang(翁誌煌); Wang, Chun-Chieh; Ho, Mon-Shu(何孟書); Yen, Li-Ting; Chen, Jian-Yu; Gaybullaev, Gulomjon; Poonpakdee, Chakkrit; Lin, Yao-Tung(林耀東)* |
| DOI | 10.1016/j.cej.2020.127880 |
| 中文摘要 | 可見光驅動光滅活系統下細菌損傷的機制。 原子力顯微鏡 (AFM) 和透射 X 射線顯微鏡 (TXM) 首次用於識別生物物理特性的變化。N-TiO2可見光失活下微生物細胞的細胞高度、粗糙度、粘附和模量以及 3D 細胞結構變化。 結果表明，光照射後立即發生 N-TiO2 的細胞粘附，然後 N-TiO2 顆粒穿透細胞膜，並在光催化失活的對數期開始時使細胞結構變形。 外層細胞的破壞導致細胞K+洩漏和脂質過氧化，最終導致細胞密度嚴重下降。 AFM 和 TXM 提供了在納米尺度上對單細胞和納米材料之間相互作用的直接觀察，對於細胞損傷的早期診斷很有用。 |
| 英文摘要 | The mechanism of bacterial damage under visible-light-driven photo-inactivation system was attempted. Atomic force microscopy (AFM) and transmission X-ray microscopy (TXM) were firstly used to identify changes in biophysical properties, such as cellular height, roughness, adhesion, and modulus, and 3D cellular structure of microbial cells under visible-light-responsive N-TiO2 inactivation. Results revealed that the cell adhesion of N- TiO2 occurred immediately upon light irradiation then N-TiO2 particles penetrated the cell membrane, and deformed the cellular structure at the beginning of log phase under photocatalytic inactivation. The destruction of the outer cellular caused leakage of cellular K+ and lipid peroxidation, which ultimately brought about severe decrease in cell density. AFM and TXM provided direct observations on interactions between single cell and nano-materials at the nanoscale, which was useful for the early diagnosis of cell damage. |
| 發表成果與本中心研究主題相關性 | 農業新穎材料在農產品保鮮開發、應用與機理 |
