生態農業：農業溫室氣體減量【生命科學系/黃盟元副教授】
| 論文篇名 | 英文：Comparisons between yellow and green leaves of sweet potato cultivars in chlorophyll fluorescence during various temperature regimes under high light intensities 中文：高光及不同溫度下黃葉及綠葉甘藷葉綠素螢光的比較 |
| 期刊名稱 | Scientia Horticulturae |
| 發表年份,卷數,起迄頁數 | 2021, 288, no.110335 |
| 作者 | Lin, Hsin-Hung; Lin, Kuan-Hung; Jiang, Jhong-Ying; Wang, Ching-Wen; Chen, Chung-, I; Huang, Meng-Yuan(黃盟元)*; Weng, Jen-Hsien* |
| DOI | 10.1016/j.scienta.2021.110335 |
| 中文摘要 | 本研究觀察兩種甘藷 (Ipomoea batatas L. Lam) 栽培品種 'Taoyaun 2'（綠葉）和 'CN1927'（黃葉）的葉綠素螢光 (ChlF) 在不同光強度和溫度下的變化。甘藷枝條在溫室盆栽中生長 4 週，然後移至暗室過夜，在 10、25 和 35°C的環境使用 1,000 和 2,000 μmol m-2 s-1 的光強度照射植物 30 分鐘，並進行 30 分鐘的暗恢復期， ChlF 在光照前立即測量，光照期間每 2~5 分鐘測量一次，直到暗恢復結束。結果顯示葉綠素的積累影響葉片顏色。在不同溫度及光強度下甘藷的 ChlF 指數Fv/Fm、Fv‘/Fm’和非光化學螢光消散 (NPQ) 隨處理時間變化，35°C 1,000 μmol m-2 s-1光照處理，綠葉甘藷比黃葉甘藷有較高的 PS II (E) 和 PSII 天線吸收光的比例 (P) ，但在熱消散的比例(D)較低。隨著溫度升高，所有植物的 P 值增加，但 D 值降低。在所有溫度下，2000 μmol m-2 s-1 光照處理時，綠葉qE% 顯著高於黃葉，而黃葉qI% 顯著高於綠葉。此外， NPQ與Fv’/Fm’和ΔF/Fm’呈顯著負相關，而綠葉甘藷D與P呈顯著負相關， E和P呈顯著正相關。光能的利用及其光保護的調節機制是增加生物能量和糧食作物產量的關鍵，根據光強度利用 ChlF 參數對作物進行有效管理，可以更輕易了解在不同溫度和光強度組合下的蔬菜光合特性。 |
| 英文摘要 | The objective of this work was to study changes in the chlorophyll fluorescence (ChlF) components of two sweet potatoes (Ipomoea batatas L. Lam) cultivars ‘Taoyaun 2’ (green leaf) and ‘CN1927’ (yellow leaf) in response to varying light intensities and temperatures. Cuttings were grown in potting soil under a controlled greenhouse for 4 weeks and moved to a dark room overnight. Plants were then illuminated with 1,000 and 2,000 μmol m-2 s-1 of photosynthetic photon flux density (PPFD) at 10, 25, and 35℃ for 30 min with a cool light source, followed by a 30 min dark recovery period at each measured temperature. ChlF was measured immediately before illumination and every 2~5 min during illumination until the end of dark recovery. All measurements were performed at 2 min intervals during the temperature exposure period. The accumulation of chlorophyll affected the leaf colors, and those ChlF indices of yellow- and green-leaved plants treated under various temperatures in response to various light intensities over time, showing wide variations in maximal chlorophyll fluorescence Fv/Fm (in darkness), Fv’/Fm’ (in the light), and non-photochemical fluorescence quenching (NPQ) levels. Moreover, green-leaved plants showed greater the fraction of excess energy in PS II (E) and fraction of light absorbed in PSII antennae (P) than yellow-leaved plants, but lower the fraction of light absorbed in PSII antennae that is dissipated thermally (D) than yellow-leaved plants at 35℃ under 1,000 μmol m-2 s-1. As temperature increased, P values of all plants increased but D values decreased. The values of qE% in green leaves were significantly higher than those in yellow leaves, whereas qI% in yellow leaves was significantly higher than in green leaves under 2,000 μmol m-2 s-1 at all temperatures. There were significant and negative correlations of NPQ with Fv’/Fm’ and ΔF/Fm’, and of D with P of green leaves, but significant and positive relationship was detected between E and P of green leaved plants. Manipulation of light harvesting and its regulation by photo-protection might be a key to increase bioenergy and food crop yields. The effective management of ChlF parameters in response to the light intensity provides a better understanding of the photosynthetic characteristics of leafy vegetables grown in controlled environments with various combinations of varying temperatures and high light intensities. |
| 發表成果與本中心研究主題相關性 | 生態農業：農業溫室氣體減量 (利用非破壞性方式測量生理指標監測植物光合固碳能力，提供未來農業生產力及碳封存能力參考估算指標) |
