Arabidopsis RAB8A, RAB8B, and RAB8D proteins interact with several RTNLB proteins and are involved in the Agrobacterium tumefaciens infection process | IDCSA, NCHU

Arabidopsis RAB8A, RAB8B, and RAB8D proteins interact with several RTNLB proteins and are involved in the Agrobacterium tumefaciens infection process 2021-07-14

Recycling Agriculture: Recycling of Agricultural Waste【Department of Life Sciences / Hwang, Hau-Hsuan / Associate Professor】
循環農業：農業廢棄物再資源化【生命科學系/黃皓瑄副教授/優聘教師】

論文篇名	英文：Arabidopsis RAB8A, RAB8B, and RAB8D proteins interact with several RTNLB proteins and are involved in the Agrobacterium tumefaciens infection process. 中文：阿拉伯芥的RAB8A、8B、8D蛋白質可與植物中的RTNLB蛋白質結合並進而參與農桿菌感染過程
期刊名稱	Plant and Cell Physiology
發表年份,卷數,起迄頁數	2021, pcab112
作者	Huang, Fan-Chen; Chi, Shin-Fei; Chien, Pei-Ru; Liu, Yin-Tzu; Chang, Hsin-Nung; Lin, Choun-Sea; Hwang, Hau-Hsuan(黃皓瑄)*
DOI	10.1093/pcp/pcab112
中文摘要	已知植物的AtRAB8蛋白質是GTPase可參與細胞內膜運輸過程，且有五個家族成員：AtRAB8A-8E。以yeast two-hybrid、GST pull-down、BFC檢測得知RAB8A、8B、8D蛋白質可與農桿菌的VirB2蛋白質及植物中RTNLB蛋白質結合。另外植物AtRAB8B蛋白質可與農桿菌VirB2結合，且其大量表現轉植株較易被農桿菌感染。且AtRAB8A、8B及8D突變株較不易被農桿菌和P. syringae pv. tomato DC3000 (Pst DC3000)感染，及AtRAB8大量表現轉植株則較易被農桿菌和Pst DC3000感染之效率，研究結果顯示AtRAB8蛋白質極有可能參與在植物與農桿菌或Pst DC3000交互作用過程。本研究結果可幫助我們了解植物的AtRAB8蛋白質，如何參與細菌感染植物的過程。
英文摘要	Arabidopsis thaliana small GTP-binding proteins, AtRAB8s, associate with the endomembrane system and modulate tubulovesicular trafficking between compartments of the biosynthetic and endocytic pathways. There are 5 members in Arabidopsis, namely AtRAB8A-8E. Yeast two-hybrid assays, bimolecular fluorescence complementation (BiFC) assays, and glutathione-S-transferase (GST) pull-down assays showed that RAB8A, 8B, and 8D interacted with several membrane-associated reticulon-like (AtRTNLB) proteins in yeast, plant cells, and in vitro. Furthermore, RAB8A, 8B, and 8D proteins showed interactions with the Agrobacterium tumefaciens virulence protein, VirB2, a component of a Type IV secretion system (T4SS). A. tumefaciens uses a T4SS to transfer T-DNA and Virulence proteins to plants, which causes crown gall disease in plants. The Arabidopsis rab8A, rab8B, and rab8D single mutants showed decrease levels of Agrobacterium-mediated root and seedling transformation, while the RAB8A, 8B, and 8D overexpression (O/E) transgenic Arabidopsis plants were hypersusceptible to A. tumefaciens and Pseudomonas syringae infections. RAB8A-8E transcripts accumulated differently in roots, rosette leaves, cauline leaves, inflorescence, and flowers of wild-type plants. In summary, RAB8A, 8B, and 8D interacted with several RTNLB proteins and participated in A. tumefaciens and P. syringae infection processes.
發表成果與本中心研究主題相關性	此篇研究結果顯示植物中的AtRAB8蛋白質可與農桿菌的T線毛蛋白質和植物的RTNLB蛋白質結合，且將rab8基因突變或大量表現時被農桿菌和Pst DC3000細菌感染的效率皆受影響。本研究結果可幫助我們了解植物的AtRAB8蛋白質，如何參與植物和細菌感染過程。此研究成果可幫助我們建構更有效率之植物轉殖系統。