循環農業：農業廢棄物再資源化【生科系黃介辰特聘教授】
論文篇名 英文：Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories.
中文：分析具備合成類胡蘿蔔素之基因工程酵母菌其抗壓力能力與作為細胞工廠基礎架構之潛力
期刊名稱 MICROBIAL CELL FACTORIES
發表年份,卷數,起迄頁數 2019,18(1), No.155
作者 Liu, Hsien-Lin（劉憲霖）;Chang, Jui-Jen;Thia, Caroline;Lin, Yu-Ju;Lo, Shou-Chen;Huang, Chieh-Chen(黃介辰)*;Li, Wen-Hsiung
DOI 10.1186/s12934-019-1205-y
中文摘要 背景: 對於利用基因工程修改微生物生產其所不曾擁有之物質，將對菌種本生造成衝擊性的壓力，因此我們將導入類胡蘿蔔素合成路徑之酵母菌(Kluyveromyces marxianus)，並觀察其是否能增強酵母菌本身抗壓性，而成為細胞工廠基礎架構之潛力。
結果: 類胡蘿蔔素為一群是具有高度營養價值及市場潛力之抗氧化物質，我們觀察發現導入類胡蘿蔔素合成路徑之酵母菌能有效提升宿主移除本身自由基的能力，並使其能夠耐受更高濃度的酒精，因此也使此改造菌種本身生質酒精產量之提升。而對於生質酒精之生產製程，我們也發現此菌種更能提升在製程中其他有毒物質之抗性，像是糠醛、重金屬砷及異丁醇。除此之外，我們也發現此菌種抗酒精之能力，更能應用在天然藥物生物轉換製成，提升疏水性前驅物因使用酒精最為導入溶劑的生物容忍瓶頸，此研究顯示也能有效提升紫杉醇(paclitaxel)前驅物10-deacetylbaccatin III的耐受性，此優勢將提升此概念在製藥產業的應用。
總結: 類胡蘿蔔素合成路徑之基應工程菌種證實能提升對於多種物質的耐受性，因而此概念更能作為細胞工廠基礎架構之潛力，並應用於共同生產類胡蘿蔔素與其他目標物質，達到生物精煉低成本高效益之概念。
英文摘要 Background A microorganism engineered for non-native tasks may suffer stresses it never met before. Therefore, we examined whether a Kluyveromyces marxianus strain engineered with a carotenoid biosynthesis pathway can serve as an anti-stress chassis for building cell factories. Results Carotenoids, a family of antioxidants, are valuable natural products with high commercial potential. We showed that the free radical removal ability of carotenoids can confer the engineered host with a higher tolerance to ethanol, so that it can produce more bio-ethanol than the wild type. Moreover, we found that this engineered strain has improved tolerance to other toxic effects including furfurals, heavy metals such as arsenate (biomass contaminant) and isobutanol (end product). Furthermore, the enhanced ethanol tolerance of the host can be applied to bioconversion of a natural medicine that needs to use ethanol as the delivery solvent of hydrophobic precursors. The result suggested that the engineered yeast showed enhanced tolerance to ethanol-dissolved hydrophobic 10-deacetylbaccatin III, which is considered a sustainable precursor for paclitaxel (taxol) bioconversion. Conclusions The stress tolerances of the engineered yeast strain showed tolerance to several toxins, so it may serve as a chassis for cell factories to produce target products, and the co-production of carotenoids may make the biorefinary more cost-effective.