循環農業：農業有機廢棄物生物處理再利用【土壤環境科學系/沈佛亭教授】
| 論文篇名 | 英文：Enhancing symbiotic nitrogen fixation and soybean growth through co-inoculation with Bradyrhizobium and Pseudomonas isolates 中文：共接種Bradyrhizobium與Pseudomonas分離株提升大豆共生固氮作用與其生長 |
| 期刊名稱 | SUSTAINABILITY |
| 發表年份,卷數,起迄頁數 | 2021, 13, 11539 |
| 作者 | Tu, Ting-Chen; Lin, Shih-Han; Shen, Fo-Ting(沈佛亭)* |
| DOI | 10.3390/su132011539 |
| 中文摘要 | 於茶園強酸性紅土中篩選具耐酸特性之綠肥大豆根瘤菌與非根瘤菌，進一步探討接種有益細菌對綠肥大豆共生固氮及其生長之影響。研究結果顯示Bradyrhizobium diazoefficiens可誘導綠肥大豆根系形成根瘤並具共生固氮活性，將上述根瘤菌與具促進植物生長潛力之非根瘤菌Pseudomonas共同接種至種植於紅土盆栽之綠肥大豆，與未接種細菌或僅接種根瘤菌的處理比較，發現共同接種兩細菌可明顯提高綠肥大豆共生固氮活性、根與地上部鮮重與乾重及植體中之營養元素氮、磷、鉀、鈣、鎂、硫、錳、銅、鋅含量。 |
| 英文摘要 | The present study was undertaken to explore acidotolerant rhizobial and non-rhizobial bacteria associated with root nodules of soybean (Glycine max L.). Genotypic and phenotypic characterization regarding nitrogen fixation, nodulation and other potentially plant growth-promotion traits were performed in several isolates. Influences of bacterial inoculation on symbiotic nitrogen fixation and soybean growth were evaluated through flask and pot experiments in a greenhouse. The highest nitrogen-fixing activity was recorded in isolate Bra6, which was closely related to Bradyrhizobium diazoefficiens based on 16S rDNA, nifH, and nodD gene sequences. All the non-rhizobial Pseudomonas isolates possessed multiple plant growth-promoting traits, with various hydrolytic patterns toward plant constituents. In sterile water agar-containing flasks, Bra6 + Pse2 treatment significantly (p < 0.05) increased the number of nodules, fresh weight, and dry weight of both root and shoot. This also led to the increment of most of the nutrients in the soybean plant compared with the uninoculated control or sole inoculation of Bra6. In non-sterile strongly acidic soil-containing pots, co-inoculation with Bra6 and various Pseudomonas isolates showed distinctively positive effects on symbiotic nitrogen fixation and soybean growth. The highest symbiotic nitrogen-fixing activity; root and shoot biomass; as well as N, P, K, Ca, Mg, S, Mn, Cu, and Zn contents of soybean plant were observed in Bra6 + Pse2 treatment. Synergistic symbiosis occurred through co-inoculation with Bradyrhizobium and Pseudomonas isolates, which further enhanced nutrients’ acquisition and growth of soybean in the strongly acidic soils. |
| 發表成果與本中心研究主題相關性 | 本研究旨在探討共接種根瘤菌與非根瘤菌對綠肥大豆結瘤與共生固氮之影響，進而評估共接種菌群對大豆生長與植體營養元素含量提升之功效，研究成果有助於大豆產量提升與農田土壤永續發展。 |
