循環農業：農業廢棄物再資源化【生命科學系/黃介辰特聘教授】
| 論文篇名 | Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture |
| 期刊名稱 | SCIENTIFIC REPORTS |
| 發表年份,卷數,起迄頁數 | 2021, 11(1): 14552 |
| 作者 | Lo, Shou-Chen*; Yang, Chia-Yin; Mathew, Dony Chacko; Huang, Chieh-Chen(黃介辰)* |
| DOI | 10.1038/s41598-021-94101-y |
| 英文摘要 | Kluyveromyces marxianus is a yeast that could be identified from kefir and can use a broad range of substrates, such as glucose and lactate, as carbon sources. The lactate produced in kefir culture can be a substrate for K. marxianus. However, the complexity of the kefir microbiota makes the traits of K. marxianus difficult to study. In this research, we focused on K. marxianus cultured with lactate as the sole carbon source. The optimal growth and released protein in lactate culture were determined under different pH conditions, and the LC-MS/MS-identified proteins were associated with the tricarboxylic acid cycle, glycolysis pathway, and cellular stress responses in cells, indicating that autolysis of K. marxianus had occurred under the culture conditions. The abundant glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) was cocrystallized with other proteins in the cell-free fraction, and the low transcription level of the GAP1 gene indicated that the protein abundance under autolysis conditions was dependent on protein stability. These results suggest that lactate induces the growth and autolysis of K. marxianus, releasing proteins and peptides. These findings can be fundamental for K. marxianus probiotic and kefir studies in the future. |
| 發表成果與本中心研究主題相關性 | 應用合成生物學建構固碳酵母菌應用於生質能之生產，有助於發展農業廢棄物再利用。 |
