循環農業：農業廢棄物再資源化【生命科學系/黃介辰特聘教授】
| 論文篇名 | Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system |
| 期刊名稱 | PLOS ONE |
| 發表年份,卷數,起迄頁數 | 2021, 16(3): e0247135 |
| 作者 | Dung Minh Ha-Tran; Lai, Rou-Yin; Trinh Thi My Nguyen; Huang, Eugene; Lo, Shou-Chen*; Huang, Chieh-Chen(黃介辰)* |
| DOI | 10.1371/journal.pone.0247135 |
| 英文摘要 | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes play important roles in CO2 fixation and redox balancing in photosynthetic bacteria. In the present study, the kefir yeast Kluyveromyces marxianus 4G5 was used as host for the transformation of form I and form II RubisCO genes derived from the nonsulfur purple bacterium Rhodopseudomonas palustris using the Promoter-based Gene Assembly and Simultaneous Overexpression (PGASO) method. Hungateiclostridium thermocellum ATCC 27405, a well-known bacterium for its efficient solubilization of recalcitrant lignocellulosic biomass, was used to degrade Napier grass and rice straw to generate soluble fermentable sugars. The resultant Napier grass and rice straw broths were used as growth media for the engineered K. marxianus. In the dual microbial system, H. thermocellum degraded the biomass feedstock to produce both C-5 and C-6 sugars. As the bacterium only used hexose sugars, the remaining pentose sugars could be metabolized by K. marxianus to produce ethanol. The transformant RubisCO K. marxianus strains grew well in hydrolyzed Napier grass and rice straw broths and produced bioethanol more efficiently than the wild type. Therefore, these engineered K. marxianus strains could be used with H. thermocellum in a bacterium-yeast coculture system for ethanol production directly from biomass feedstocks |
| 發表成果與本中心研究主題相關性 | 應用合成生物學建構固碳酵母菌應用於生質能之生產，有助於發展農業廢棄物再利用。 |
