新穎材料農業：農業新穎材料在植物保健開發、應用與機理【植物病理學系/黃振文教授；洪爭坊助理教授】
| 論文篇名 | 英文：Identification and Characterization of a Multifunctional Biocontrol Agent, Streptomyces griseorubiginosus LJS06, Against Cucumber Anthracnose 中文：多功能生物防治鏈黴菌Streptomyces griseorubiginosus LJS06的鑑定與其防治胡瓜炭疽病的效果分析 |
| 期刊名稱 | FRONTIERS IN MICROBIOLOGY |
| 發表年份,卷數,起迄頁數 | 2022, 13, 923276 |
| 作者 | Chai, Chien Hao; Hong, Cheng-Fang(洪爭坊)*; Huang, Jenn-Wen(黃振文) |
| DOI | 10.3389/fmicb.2022.923276 |
| 英文摘要 | Twenty-eight bacterial strains isolated from Chinese herb extracts, beer fermentation waste, and raw oyster shells were evaluated for their efficacy in controlling cucumber anthracnose. Four bacterial strains, namely TG01, TG02, LJS06, and LJS08, were found to effectively reduce the mycelial growth of Colletotrichum orbiculare COC3 on PDA media. Spraying or drenching LJS06 spore suspension before inoculation significantly (p < 0.05) reduced disease severity; thus, LJS06 was subject to further characterization. On the basis of the morphological, physiological, and biochemical characteristics and a multilocus sequence analysis of partial 16S rRNA, atpD, rpoB, and trpB genes, LJS06 was identified to be Streptomyces griseorubiginosus (Ryabova and Preobrazhenskaya) Pridham et al. Physiological and biochemical tests revealed that S. griseorubiginosus LJS06 can produce amylase, cellulase, chitinase, protease, siderophore, polyamines, and indole-3-acetic acid. Thus, a culture filtrate of LJS06 (specifically SL06) was formulated and evaluated for its efficacy against conidial germination, appressorium formation, and anthracnose management. Diluted SL06 was found to significantly (p < 0.05) inhibit conidial germination and appressorium formation, which can be attributed to impaired membrane integrity, accumulated reactive oxygen species (ROS), and impaired energy metabolism in the conidia. In addition, the spraying and drenching of diluted SL06 before inoculation consistently and significantly (p < 0.05) reduced anthracnose severity. These results jointly suggest that S. griseorubiginosus LJS06 can aid in the management of cucumber anthracnose by directly inhibiting conidial function and priming the plant defense system. |
| 發表成果與本中心研究主題相關性 | 本研究由農業副產物中篩選並鑑定具有多功能的生物防治鏈黴菌Streptomyces griseorubiginosus LJS06。該菌株的培養濾液可能影響胡瓜炭疽病菌的分生孢子細胞膜通透性，導致孢子內的活性氧累積，進而抑制胡瓜炭疽病的孢子發芽。LJS06菌株除了具有促進植株生長的作用，其培養稀釋濾液可直接施用於胡瓜葉片以降低炭疽病為害，施用於土壤中時，則可預啟胡瓜的抗病反應，達到降低胡瓜炭疽病的效果。本項成果未來可開發作為農藥的替代資材，亦可進一步配合農業副產物或循環利用栽培介質，開發成為抑病介質，達到循環利用資源與減碳減廢的目的。 |
