循環農業：農業有機廢棄物生物處理再利用【土環系沈佛亭副教授】
論文篇名 英文：Screening of rice endophytic biofertilizers with fungicide tolerance and plant growth-promoting characteristics
中文：具農藥耐受性且可促進水稻生長之內生細菌生物肥料開發
期刊名稱 Sustainability
發表年份,卷數,起迄頁數 2019, 11, 1133
作者 Shen, Fo-Ting (沈佛亭); Yen, Jui-Hing; Liao, Chien-Sen; Chen, Wen-Ching*; Chao, Yi-Ting
DOI 10.3390/su11041133
中文摘要 本研究旨在開發具農藥耐受性且可促進水稻生長之內生細菌生物肥料。研究中先將水稻幼苗分別種植於含1倍、5倍與10倍農藥推薦劑量之三種殺真菌劑(etridiazole, metalaxyl和tricyclazole)土壤中，待植株生長後分離幼苗根系中的內生細菌。進一步利用16S rDNA序列分析得知各分離株之分類地位，再針對與植物生長促進相關之特性包括固氮、溶磷與IAA生成能力進行探討。本研究自水稻幼苗根系中共分離17株細菌，其中5株菌Rhizobium larrymoorei E2, Bacillus aryabhattai E7, Bacillus aryabhattai MN1, Pseudomonas granadensis T6和Bacillus fortis T9為同時具有固氮及生成IAA能力表現較佳者。除T9菌株外其餘4株菌皆可耐受兩種以上的試驗農藥。分別接種上述5株內生菌至水稻幼苗後，結果顯示皆可促進水稻之生長。其中進一步分析得知當培養基中的色胺酸濃度提高時，Bacillus aryabhattai MN1生成IAA之量亦可隨之提高，此菌株亦可耐受農藥etridiazole與metalaxyl，是極具潛力之生物肥料菌株。
英文摘要 The application of pesticides is unavoidable in conventional agricultural practice. To develop effective biofertilizers, bacterial strains with both pesticide tolerance and plant growth-promoting (PGP) traits were isolated for further testing. Seedlings of rice (Oryza sativa) were planted in soil with 1, 5, or 10 times the recommended rates of the fungicides etridiazole, metalaxyl, and tricyclazole. Endophytic bacteria were isolated from roots of rice seedlings. The bacterial 16S rDNA sequences and related PGP characteristics including potential nitrogen fixation, phosphorus-solubilizing and indole acetic acid (IAA) production ability were further examined. In all, 17 different strains were obtained from rice seedling roots; five strains with both nitrogen fixation potential and IAA production ability included Rhizobium larrymoorei E2, Bacillus aryabhattai E7, Bacillus aryabhattai MN1, Pseudomonas granadensis T6, and Bacillus fortis T9. Except for T9, all strains could tolerate two or more fungicides. We inoculated rice roots with the endophytic bacteria and all conferred rice growth-promoting ability. Bacillus aryabhattai MN1 was further tested and showed high tryptophan dose-dependent IAA production ability, tolerance towards etridiazole and metalaxyl application and should be considered a potential bacterial biofertilizer.