設施農業：智慧感測【生機系吳靖宙特聘教授】
論文篇名 英文：Effect of Poly-L-Lysine Polycation on the Glucose Oxidase/Ferricyanide Composite-Based Second-Generation Blood Glucose Sensors
中文：聚左旋賴胺酸陽離子在Glucose Oxidase/Ferricyanide 複合體的第二代血糖感測器的影響
期刊名稱 Sensors
發表年份,卷數,起迄頁數 2019, 19, 1448
作者 Ming-Jie Lin, Ching-Chou Wu and Ko-Shing Chang
DOI 10.3390/s19061448
中文摘要 第二代血糖感測器為目前糖尿病患者商業化血糖感測器的主流。網版印刷碳電極(SPCE)是最多被使用的血糖測試片基才。本研究使用帶正電的親水性聚左旋賴胺酸(aPLL) 陽離子包埋帶負電之葡萄糖氧化酵素(GOx)與ferricyanide (FIC)在SPCE上以構成第二代葡萄糖感測器。aPLL的修飾可促進FIC的氧化還原動力學。0.5 mM GOx, 99.5 mM FIC 與5 mM aPLL的配方可使測試片在2.8-27.58 mM間有良好的線性度，其檢測極限為2.3 mM。而且以5 mM  aPLL修飾的試片在50C的老化測試實驗中顯示具有好的長期穩定度以維持GOx的活性。
英文摘要 Second-generation glucose biosensors are presently the mainstream commercial solution for blood glucose measurement of diabetic patients. Screen-printed carbon electrodes (SPCEs) are the most-used substrate for glucose testing strips. This study adopted hydrophilic and positively charged a-poly-L-lysine (aPLL) as the entrapment matrix for the immobilization of negatively charged glucose oxidase (GOx) and ferricyanide (FIC) on SPCEs to construct a disposable second-generation glucose biosensor. The aPLL modification is shown to facilitate the redox kinetics of FIC and ferrocyanide on the SPCEs. The SPCEs coated with 0.5 mM GOx, 99.5 mM FIC, and 5 mM aPLL had better sensitivity for glucose detection due to the appreciable effect of protonated aPLL on the promotion of electron transfer between GOx and FIC. Moreover, the SPCEs coated with 0.5 mM GOx, 99.5 mM FIC, and 5 mM aPLL were packaged as blood glucose testing strips for the measurement of glucose-containing human serum samples. The glucose testing strips had good linearity from 2.8 mM to 27.5 mM and a detection limit of 2.3 mM. Moreover, the 5 mM  aPLL-based glucose testing strips had good long-term stability to maintain GOx activity in aging tests at 50
C.