循環農業：農業廢棄物再資源化—研發低碳農業技術【食品暨應用生物科技學系/蔣恩沛教授】
| 論文篇名 | 英文：Association of Metallothionein 2A rs10636 with low mean corpuscular volume (MCV), low mean corpuscular haemoglobin (MCH) in healthy Taiwanese 中文：金屬硫蛋白 2A rs10636 與台灣健康群體平均紅血球體積及低平均細胞血紅素之關聯性 |
| 期刊名稱 | Scientific Reports |
| 發表年份,卷數,起迄頁數 | 2023, 13 (1): 1292 |
| 作者 | Chen, Rong-Fu; Chen, Po-Ming; Pan, Chau-Shiung; Huang, Chieh-Cheng(黃介辰); Chiang, En-Pei Isabel(蔣恩沛)* |
| DOI | 10.1038/s41598-022-27304-6 |
| 中文摘要 | 人類金屬硫蛋白-2A (MT2A) 參與金屬離子平衡、排毒、氧化壓力降低及免疫防禦等反應。它在細胞和組織受傷時減少重金屬離子和活性氧物質。MT2A 基因上的單核苷酸多態性與多種人類疾病相關。本研究旨在闡明 MT2A 基因型與臨床、生化和分子特徵間之關聯性。檢測健康受試者並 MT2A 基因內常見之等位基因SNP (rs28366003 及 rs10636) 之臨床、生化及分子特性。MT2A rs10636 的基因型分佈符合 Hardy-Weinberg 平衡。使用ANOVA分析與 Tukey 事後檢驗發現 MT2A rs10636及rs28366003 與平均紅血球體積和平均細胞血紅素下降之間的顯著關聯。進一步通過網路基因表現譜數據動態分析數據庫，驗證紅血球生成、膽固醇合成、血小板生成、胰島素等基因與 MT2A 之間的相關性。結果顯示缺氧誘導因子 1α、促紅血球生成素、脂蛋白酶及卵磷脂-膽固醇酰基轉移酶mRNA表現與MT2A顯著相關。這些結果顯示 MT2A rs10636 與 rs28366003 的基因多型性可能是影響紅血球生成之重要風險因子。 |
| 英文摘要 | Human metallothionein-2A (MT2A) protein participates in metal homeostasis, detoxification, oxidative stress reduction, and immune defense. It decreases heavy metal ions and reactive oxygen species (ROS) during injury of cells and tissues. The single nucleotide polymorphisms at the MT2A gene have been associated in various human diseases including cancer. The current study aimed to elucidate associations between MT2A genotypes with the clinical, biochemical, and molecular characteristics that potentially related to lowered MT2A expression. One hundred and forty-one healthy Taiwanese subjects were enrolled from Changhua Show-Chwan Memorial Hospital. Clinical, biochemical and molecular characteristics including the frequent minor allele SNPs, rs28366003 and rs10636, within the MT2A gene were determined. The genotype distribution of MT2A rs10636 fits the Hardy-Weinberg equilibrium. The significant associations with gradually decline of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were identified with MT2A rs10636 and rs28366003 using analysis of variance (ANOVA) with Tukey's analysis as a post hoc test. We further validated the correlations between the expressions of genes in erythropoiesis, cholesterol synthesis, platelet synthesis, insulin with MT2A using the web-based Gene Expression Profiling Interactive Analysis (GEPIA) databases. The results revealed that hypoxia-inducible factor 1α (HIF-1α), erythropoietin (EPO), lipoprotein lipase (LPL), and lecithin-cholesterol acyltransferase (LCAT) mRNA expression are significantly correlated with MT2A mRNA expression. In conclusion, these results suggested that genetic variations of MT2A rs10636 and rs28366003 might be an important risk factor for erythropoiesis in the Taiwanese general population. |
| 發表成果與本中心研究主題相關性 | 代謝檢驗技術及資訊數據庫之分析與應用 |
