Recycling Agriculture: Agricultural Waste Recycling: Research and Development of Low-Carbon Agricultural Technologies【Department of Life Sciences / Huang, Chieh-Chen / Professor】
循環農業：農業廢棄物再資源化—研發低碳農業技術【生命科學系/黃介辰教授】
論文篇名 英文：Characterization of the Pyrroloquinoline Quinone Producing Rhodopseudomonas palustris as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions
期刊名稱 International Journal of Molecular Sciences
發表年份,卷數,起迄頁數 2023, 24(18), 14080
作者 Lo, Shou-Chen; Tsai, Shang-Yieng; Chang, Wei-Hsiang; Wu, I-Chen; Sou, Nga-Lai; Hung, Shih-Hsun Walter; Chiang, En-Pei Isabel(蔣恩沛); Huang, Chieh-Chen(黃介辰)*; Lin, Fucheng
DOI 10.3390/ijms241814080
中文摘要 Rhodopseudomonas palustris是一種紫色非硫細菌（PNSB），目前已經證明一些品系能夠促進植物生長。然而，我們對這些PNSB的作用機制所知仍然有限。從基因體資料可知，R. palustris具有生產吡咯喃喹喔醌（PQQ）的潛力。而PQQ已知在刺激植物生長、促進磷溶解和作為活性氧自由基清除方面發揮著關鍵作用。然而，目前尚不確定生長條件是否會影響R. palustris的PQQ生合成與其他特性。本研究發現，與乙酸培養條件相比，R. palustris在自營培養條件下的PQQ合成相關基因有更高的表現。此外，磷溶解和鐵載體活性的模式也有相同的趨勢，而這兩者都被認為是有助於植物生長的。然而，這些PNSB培養條件在阿拉伯芥生長實驗中並未出現顯示差異，這表明除了PQQ含量之外，可能還有其他因素影響植物生長。此外，從阿拉伯芥中分離出的內生細菌種類會依PNSB培養條件的不同而有差異。這些發現意味著，根據PNSB的生長條件，它可能會與許多土壤細菌相互作用，並促進它們進入植物之中。
英文摘要 Rhodopseudomonas palustris is a purple non-sulfide bacterium (PNSB), and some strains have been proven to promote plant growth. However, the mechanism underlying the effect of these PNSBs remains limited. Based on genetic information, R. palustris possesses the ability to produce pyrroloquinoline quinone (PQQ). PQQ is known to play a crucial role in stimulating plant growth, facilitating phosphorous solubilization, and acting as a reactive oxygen species scavenger. However, it is still uncertain whether growth conditions influence R. palustris’s production of PQQ and other characteristics. In the present study, it was found that R. palustris exhibited a higher expression of genes related to PQQ synthesis under autotrophic culture conditions as compared to acetate culture conditions. Moreover, similar patterns were observed for phosphorous solubilization and siderophore activity, both of which are recognized to contribute to plant-growth benefits. However, these PNSB culture conditions did not show differences in Arabidopsis growth experiments, indicating that there may be other factors influencing plant growth in addition to PQQ content. Furthermore, the endophytic bacterial strains isolated from Arabidopsis exhibited differences according to the PNSB culture conditions. These findings imply that, depending on the PNSB’s growing conditions, it may interact with various soil bacteria and facilitate their infiltration into plants.
發表成果與本中心研究主題相關性 基於開發高價生物性化合物pyrroloquinoline quinone (PQQ)，我們從基因體資料分析出光合細菌具有生產PQQ的潛力。而在測試生產PQQ條件時，我們發現不同培養條件的光合細菌除了產生PQQ的能力具差異外，對植物內生菌的菌相也會產生影響。這對植物益生菌的相關研究提供了新的分析面向。