Facility Agricultural Green Energy Development and Carbon Offset【Department of Bio-industrial Mechatronics Engineering / Wu, Ching-Chou / Professor】
設施農業：農業綠能開發與碳匯補償【生物產業機電工程學系/吳靖宙教授】
論文篇名 英文：Application of aminobenzoic acid electrodeposited screen-printed carbon electrode in the beta-amyloid electrochemical impedance spectroscopy immunoassay
中文：氨基苯甲酸電鍍於網版印碳電極在β-澱粉樣品的電化學阻抗免疫分析中的應用
期刊名稱 Talanta
發表年份,卷數,起迄頁數 2023, 254, 124154
作者 Wang, Ping-Ghun; Li, Bor-Ran; Wang, Yu-Lin; Wu, Ching-Chou(吳靖宙); Chen, Jung-Chih*
DOI 10.1016/j.talanta.2022.124154
中文摘要 阿茲海默病（AD）是重要的神經退行性疾病之一，在現代老齡化社會中，它已成為人們需要解決的問題。導致AD的病因因素中，β澱粉樣蛋白（Aβ）是最重要的因素之一。它可以形成斑塊聚集在神經元中並干擾信號傳遞。本研究基於電化學系統和網版印刷碳電極（SPCE），並結合預處理、電沉積、電化學阻抗譜（EIS）和抗體固定製作出可檢測不同濃度的Aβ生物感測器。計算了電子轉移阻抗與Aβ濃度之間的標準曲線。在製程技術中優化了4-氨基苯甲酸（4-ABA）的電沉積條件以及抗體修飾的參數。本研究製備了更密集、均勻、穩定的4-ABA膜。該傳感器呈現出1 fg/ml至100 pg/ml的檢測範圍和3.84 fg/ml的檢測限制。本研究將製造時間縮短至3.5小時，並製備了一種無標記、低成本的Aβ免疫感測器，具有製造時間短、穩定性高、檢測範圍廣、檢測限制低和良好特異性等優點。
英文摘要 Alzheimer’s disease (AD) is one of the important neurodegenerative diseases, in the modern aging society, it has become an issue people need to work on. Of the pathogenic factor which leads to AD, beta-amyloid (Aβ) is the most important one. It can form the senile plaque which aggregates in the neuron and interrupts the signal transmission. This research is based on the electrochemical system and screen-printed carbon electrode (SPCE) incorporated with pretreatment, electrodeposition, electrochemical impedance spectroscopy (EIS), antibody, and blocking agent. This immunosensor is applied to detect the different concentrations of Aβ. The standard curve between electrical impedance and concentration of Aβ is calculated. The specificity of the immunosensor is tested. This survey optimizes the electrodeposition condition for 4-aminobenzoic acid (4-ABA) and the parameter for antibody and blocking agents. This study fabricates a more dense, uniform, and stable film of 4-ABA. This sensor presents a range of detection from 1 fg/ml to 100 pg/ml and a limit of detection to 3.84 fg/ml. This sensor can identify the isoform of Aβ. This research shortens the fabricating time to 3.5 h. This study fabricates a label-free and low-cost immunosensor for Aβ with a short fabricating time, high stability, wide range of detection, low limit of detection, and good specificity. The impedance of the carbon printed electrodes is very high and is always measured by its current but this study provides a fabrication technique for high-efficiency carbon printed electrodes for electrochemical impedance spectroscopy sensing.
發表成果與本中心研究主題相關性 該論文使用電沉積技術直接在網版印刷碳電極上修飾帶有COOH官能基的4-氨基苯甲酸（4-ABA），該4-ABA單層膜利於EDC/NHS的活化與抗體固定，論文雖使用該電極介面開發β澱粉樣蛋白的生物感測器，但該相同的電極介面更換不同的生物辨識分子，將可更快速開發出新型的電化學阻抗式免疫感測器，未來可應用在環境中重金屬、汙染源分子、細菌、動物健康指標蛋白的快速檢測。