設施農業：智慧感測【機械工程學系/吳嘉哲副教授】
| 論文篇名 | Detection of amyloid-β(1-42) aggregation with a nanostructured electrochemical sandwich immunoassay biosensor |
| 期刊名稱 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY |
| 發表年份,卷數,起迄頁數 | 2022, 10, 853947 |
| 作者 | Wang, Bing-Yu; Gu, Bien-Chen; Wang, Gou-Jen; Yang, Yuan-Han; Wu, Chia-Che(吳嘉哲)* |
| DOI | 10.3389/fbioe.2022.853947 |
| 英文摘要 | Amyloid-β(1–42) [Aβ(1–42)] oligomer accumulations are associated with physiologic alterations in the brains of individuals with Alzheimer’s disease. In this study, we demonstrate that a nanostructured gold electrode with deposited gold nanoparticles, induced via electrochemical impedance spectroscopy (EIS), may be used as an Aβ(1–42) conformation biosensor for the detection of Alzheimer’s disease. Monoclonal antibodies (12F4) were immobilized on self-assembled monolayers of the electrochemical sandwich immunoassay biosensor to capture Aβ(1–42) monomers and oligomers. Western blot and fluorescence microscopy analyses were performed to confirm the presence of Aβ(1–42) monomers and oligomers. EIS analysis with an equivalent circuit model was used to determine the concentrations of different Aβ(1–42) conformations in this study. We identified conformations of Aβ(1–42) monomers and Aβ(1–42) oligomers using probe antibodies (12F4) by employing EIS. RAβ(1−42) indicates the sum resistance of impedance measured during Aβ(1–42) immobilization. ΔR12F4 refers to the concentration of probe antibody (12F4) binding with Aβ(1–42). The concentration of Aβ(1–42) oligomer was defined as the percentage of Aβ(1–42) aggregation R12F4/RAβ(1−42). The experimental results show that the biosensor has high selectivity to differentiate Aβ(1–40) and Aβ(1–42) monomers and Aβ(1–42) oligomers and that it can detect Aβ(1–42) oligomer accurately. The linear detection range for Aβ(1–42) oligomers was between 10 pg/ml and 100 ng/ml. The limit of detection was estimated to be 113 fg/ml. |
| 發表成果與本中心研究主題相關性 | 本研究開發出一種以 ELISA 三明治法抗體抗原鍵結原理的方法配合電化學阻抗分析偵測微量的 Aβ1-42 蛋白聚集的情形。本技術可以應用於牧場廢棄物液態和固態廢棄物的檢測如固體懸浮物、重金屬濃度等。 |
