【學術亮點】Transcriptome and 2-DE proteome analyses reveal defense-associated development in the leaf galls induced by psyllids on Machilus japonica var. kusanoi
Ecological Agriculture: Reduce Greenhouse Gases of Agriculture【Department of Life Sciences / Huang, Meng-Yuan / Associate Professor】
生態農業：農業溫室氣體減量【生命科學系/黃盟元副教授】
| 論文篇名 | 英文：Transcriptome and 2-DE proteome analyses reveal defense-associated development in the leaf galls induced by psyllids on Machilus japonica var. kusanoi 中文：轉錄體與二維蛋白質體分析大葉楠葉片木蝨癭的防禦相關發育機制 |
| 期刊名稱 | Botanical Studies |
| 發表年份,卷數,起迄頁數 | 2025, 66, no.19 |
| 作者 | Shih, Tin-Han; Chen, Jian-Liang; Huang, Meng-Yuan(黃盟元)* |
| DOI | 10.1186/s40529-025-00470-2 |
| 中文摘要 | 背景： 癭為植物防禦反應與寄生昆蟲活動交互作用的結果。昆蟲誘導的生長重編程與氧化壓力反應共同塑造癭的發育，但其分子調控機制仍不清楚。 方法： 以寄生於 Machilus japonica var. kusanoi 的木蝨 (Neotrioza shuiliensis) 癭為材料，比較不同發育階段癭與健康葉的轉錄體與蛋白質體。 結果： 早期癭組織富含與細胞週期、微管運動及信號傳導相關的基因（如 CAM7、LSH6、eIF2β），並顯示強烈的活性氧 (ROS) 反應與防禦信號。成熟癭中則富集養分分解相關基因。早期癭內多酚含量升高，證實抗氧化代謝的活化。 結論： ROS 調控可能是癭發育的主要驅動因素，與生長激素訊號交互作用以調節癭形態。此研究揭示了昆蟲誘導植物防禦與生長重編程的整合機制，為理解癭形成的分子網絡提供新視角。 |
| 英文摘要 | Background Galls are abnormal plant tissues that result from the interaction between the defense responses of plants and the activities of galling herbivores. During infection, secondary metabolites are synthesized to mitigate the oxidative stress whereas growth and cellular processes in infected tissues are reprogrammed. Although the regulatory networks of growth-related phytohormones are recognized as the main component in gall formation, other factors like oxidative stress might also being critical in gall development. In this study, we focused on the spherical psyllid galls found in the leaves of Machilus japonica var. kusanoi to analyze the differences in transcript, protein content, and several metabolites between galls and leaves. Results Early gall tissues were enriched in cell cycle regulation and organization genes, suggesting processes, such as mitosis, meiosis, and microtubule-based movement. Proteins such as CAM7, LSH6, and eIF2β, associated with seed development, were highly expressed in early gall tissues. We demonstrated a significant role for ROS-related stress responses in early gall development. Higher amount of polyphenols, which are well-known antioxidants, was found in gall tissue as well. Conclusions This study provides valuable insights into the mechanisms underlying gall development and enhances our understanding of this complex process. Future research should explore the impact of ROS modulation on gall tissue development and assess phytohormone content at various psyllid larval and gall stages to elucidate the regulatory network involved in gall morphogenesis. |
| 發表成果與本中心研究主題相關性 | 此研究以多層次分子分析揭示木蝨癭形成過程中植物防禦與活性氧信號的作用機制。 結果深化對植物抗逆性與寄生互動的理解，提供作物防禦基因與抗蟲策略的理論依據。 |
