【學術亮點】Effects of plant growth regulators on Orobanche coerulescens seed germination
Ecological Agriculture: Reduce Greenhouse Gases of Agriculture【Department of Life Sciences / Huang, Meng-Yuan / Associate Professor】
生態農業：農業溫室氣體減量【生命科學系/黃盟元副教授】
| 論文篇名 | 英文：Effects of plant growth regulators on Orobanche coerulescens seed germination 中文：植物生長調節劑對列當種子發芽的影響 |
| 期刊名稱 | Botanical Studies |
| 發表年份,卷數,起迄頁數 | 2025, 66, no.30 |
| 作者 | Wang, Yu-Hsuan; Lin, Kuan-Hung; Tseng, Yu-Hsin; Chen, Cung-, I; Chen, Chang-Chang; Wang, Ching-Wen; Huang, Meng-Yuan(黃盟元)* |
| DOI | 10.1186/s40529-025-00479-7 |
| 中文摘要 | 背景：列當是傳統藥用與保健植物，與其他列當科植物相似，其種子發芽需要外源化學刺激物。本研究旨在探討在有無預處理條件下，植物生長調節劑對其種子發芽的影響。由寄生於茵陳蒿 (Artemisia capillaris) 的成熟植株收集種子，經 NaOCl 消毒後，在含有赤霉素 (GA₃)、氟呋酮 (fluridone)、諾氟拉宗 (norflurazon) 或油菜素內酯 (brassinolide) 的瓊脂上預處理，於 4–18 °C 黑暗中培養 3–7 天，再轉移至 GR24 或去離子水瓊脂上於 23 ± 2 °C 培養。未經預處理的種子則直接放置於不同物質瓊脂上培養 180 天。 結果顯示：以 TTC 染色檢測，第五天種子活性達 39.23%。氟呋酮與諾氟拉宗於條件化階段能顯著促進發芽，特別是在 18 °C、5 天條件下氟呋酮處理的種子經去離子水刺激後達最高發芽率；相反地，GR24 可能具有抑制而非刺激作用。 結論：本研究增進對該寄生植物種子發芽生理的理解，對保育與人工繁殖具有應用價值。 |
| 英文摘要 | Background Orobanche coerulescens, used in traditional medicine and as a nutraceutical, requires external chemical stimuli for seed germination, similar to other Orobanchaceae species. This study aims to explore the effects of plant growth regulators conditioning and non-conditioning on its seed germination. Seeds collected from mature plants parasitizing A. capillaris were stored at 4 °C to induce dormancy and sterilized with NaOCl. Seeds were sterilized with NaOCl and Tween 20, and preconditioned on agar with gibberellic acid (GA3), fluridone, norflurazon, or brassinolide, and incubated at 4–18 °C for 3–7 days in darkness. Afterward, seeds were transferred to GR24 or SD H2O agar, and incubated at 23 ± 2 °C. Non-conditioned sterilized seeds were directly germinated on agar with various substances and incubated for 180 days. Results Seeds exhibited a viability of 39.23% at the fifth day after TTC-staining. Fluridone and norflurazon significantly promote germination during conditioning with SD H2O or GR24. The highest germination rate occurred with fluridone-conditioned seeds at 18 °C for 5 days, followed by SD H2O stimulation. However, GR24 may act as an inhibitor rather than a stimulant. Conclusions These findings enhance our understanding of seed germination in this parasitic species, which can aid in conservation efforts. |
| 發表成果與本中心研究主題相關性 | 本研究針對臺灣瀕危寄生植物 Orobanche coerulescens 的種子發芽機制，探討植物生長調節劑與休眠解除的關聯。 其成果有助於建立寄生植物的保育與人工繁殖技術，兼顧生物多樣性維護與藥用資源永續利用。 |
