【學術亮點】The Efficacy of Orange Terpene and Bacillus mycoides Strain BM103 on the Control of Periwinkle Leaf Yellowing Phytoplasma
Technological Agriculture: Carbon Reduction and Sequestration Techniques through Precision Crop Cultivation Management【Department of Plant Pathology / Jan, Fuh-Jyh / Distinguished Professor、Huang, Tzu-Pi / Professor】
科技農業：精準作物栽培管理之減碳增匯技術研發【植物病理學系/詹富智特聘教授、黃姿碧教授】
| 論文篇名 | 英文：The Efficacy of Orange Terpene and Bacillus mycoides Strain BM103 on the Control of Periwinkle Leaf Yellowing Phytoplasma 中文：評估柑橘萜烯與蕈狀芽孢桿菌 BM103 防治日日春葉片黃化病之效果 |
| 期刊名稱 | Plant Disease |
| 發表年份,卷數,起迄頁數 | 2025, 109(3), 646-656 |
| 作者 | Hung, Jui-Chen; Huang, Tzu-Pi(黃姿碧); Huang, Jenn-Wen; Chang, Chung-Jan; Jan, Fuh-Jyh(詹富智)* |
| DOI | 10.1094/PDIS-07-24-1547-RE |
| 中文摘要 | 植物菌質體是一類絕對寄生的植物病原細菌，屬於柔膜菌綱。此類病原菌可由媒介昆蟲進行傳播，於全世界造成上百種植物病害。傳統上，透過抗生素治療受感染植株之防治方式已受到法規限制，而殺蟲劑對田間媒介昆蟲之防除效果也有限。有鑑於此，針對植物菌質體所造成之病害，開發友善環境的替代方式具有必要性。近年來，植物菌質體病害機制的研究促進了非化學防治資材的應用與探討，而過去研究顯示，萜烯類與有益微生物皆具有抗菌活性和促進系統性防禦反應的效果，然而二者用於植物菌質體病害防治之效果未明。因此，本研究將柑橘萜烯與蕈狀芽孢桿菌 BM103 做為防治資材，以莖頂組織培養系統，繁殖經由嫁接受日日春葉片黃化病菌質體感染之日日春植株，進行盆缽試驗，並評估二種資材用於植物菌質體病害防治之效果。結果顯示，柑橘萜烯乳化劑之最佳施用濃度為 1,000 ppm，而醒菌的措施有利於蕈狀芽孢桿菌 BM103 防治病害的效果。以 1,000 ppm 之柑橘萜烯乳化劑或 100 倍稀釋之蕈狀芽孢桿菌 BM103 發酵液每週澆灌，可抑制日日春葉片黃化病菌質體之族群量，且施用此二種防治資材皆可提升植物防禦反應與花器形成相關之基因表現。另外，於接種前預處理柑橘萜烯乳化劑或蕈狀芽孢桿菌 BM103 可延緩日日春葉片黃化病菌質體經由嫁接感染所產生的病徵。在盆缽試驗中，澆灌 1,000 ppm 之柑橘萜烯乳化劑無法減緩病徵表現，而蕈狀芽孢桿菌 BM103 之施用卻能降低病害嚴重程度，推測與其能夠產生吲哚乙酸、促進植物生長的特性有關。然而於 72 小時內，植物生長素調控相關基因 (CrIAA) 並未被上調，而內生孢子染色結果顯示，醒菌措施可大幅增加 BM103 活性細胞的數量，推測與其防治病害的效果有關。綜觀上述，本研究闡明了柑橘萜烯與蕈狀芽孢桿菌 BM103 作為生物防治資材，並應用於植物菌質體病害防治之潛力。往後應調整柑橘萜烯乳化劑的施用方式以增進其應用價值，並釐清蕈狀芽孢桿菌 BM103 或其代謝物與植物之間的交互作用，以深入了解其防治病害的機制。基於本研究中柑橘萜烯與蕈狀芽孢桿菌 BM103 快速誘導抗病反應的特性，此二種資材有望作為預防性藥劑或生物肥料應用於田間。未來可推廣至其他植物菌質體病害系統，以增進其實際應用價值。 |
| 英文摘要 | Phytoplasmas are obligate phytopathogenic bacteria belonging to the class Mollicutes. The pathogens, transmitted by insect vectors, are associated with hundreds of plant diseases worldwide. Because of the regulations banning the use of antibiotics and the limited efficacy of traditional disease management manners, an eco-friendly alternative is needed. Given that terpene and probiotics have antibiotic activity and the ability to induce systemic resistance, in this study, the effectiveness of orange terpene and a Bacillus mycoides strain, BM103, was evaluated in periwinkle plants infected with periwinkle leaf yellowing (PLY) phytoplasma derived from a shoot-tip tissue culture system. Weekly drenching of 1,000 ppm diluted orange terpene emulsion or preactivated strain BM103 liquid culture dilution exhibited the ability to inhibit PLY phytoplasma accumulation. The expression of the genes associated with plant defense response and flower development was upregulated after treatment. Moreover, pretreatment of orange terpene or strain BM103 delayed PLY infection via cleft-grafting inoculation. Although orange terpene did not suppress the symptoms, strain BM103 did result in a milder symptom expression that might partially attribute to its plant growth-promoting characteristics. Additionally, the preactivation of strain BM103 may contribute to its efficacy. Taken together, this research indicates that orange terpene and B. mycoides BM103, with the ability to rapidly induce plant defense responses, could potentially be developed into biologic control materials as preventive agents or biofertilizers. |
| 發表成果與本中心研究主題相關性 | 本研究將柑橘萜烯與蕈狀芽孢桿菌 BM103 做為防治資材，以莖頂組織培養系統，繁殖經由嫁接受日日春葉片黃化病菌質體感染之日日春植株，進行盆缽試驗，並評估二種資材用於植物菌質體病害防治之效果。進而促進非化學防治資材的應用與探討。 |
