【學術亮點】A plant endophytic bacterium Burkholderia seminalis strain 869T2 increases plant growth under salt stress by affecting several phytohormone response pathways
Recycling Agriculture: Agricultural Waste Recycling: Research and Development of Low-Carbon Agricultural Technologies【Department of Life Sciences / Hwang, Hau-Hsuan / Professor】
循環農業：農業廢棄物再資源化—研發低碳農業技術【生命科學系/黃皓瑄教授】
| 論文篇名 | 英文：A plant endophytic bacterium Burkholderia seminalis strain 869T2 increases plant growth under salt stress by affecting several phytohormone response pathways 中文：一株植物內生菌 Burkholderia seminalis 菌株 869T2，能夠透過調控多條植物激素反應途徑，在鹽分逆境下有效促進植物生長 |
| 期刊名稱 | Botanical Studies |
| 發表年份,卷數,起迄頁數 | 2025, 66, no.7 |
| 作者 | Hwang, Hau-Hsuan(黃皓瑄)*; Huang, Yu-Ting; Chien, Pei-Ru; Huang, Fan-Chen; Wu, Chih-Lin; Chen, Liang-Yu; Hung, Shih-Hsun Walter; Pan, I-Chun; Huang, Chieh-Chen(黃介辰)* |
| DOI | 10.1186/s40529-025-00453-3 |
| 中文摘要 | 由於全球暖化與逐漸加劇的氣候變遷，植物面臨多樣化的環境胁迫，對其生長與產量造成不利影響。植物自身已演化出多種機制以克服非生物逆境，例如鹽分逆境、乾旱以及強光壓力。然而，除了自我防禦策略之外，植物亦可藉由體內有益內生菌的幫助，協助其承受嚴苛的生長環境。先前自香根草中分離出的一株植物內生菌 Burkholderia seminalis 869T2，具備合成生長素、螯鐵素及溶解磷酸鹽的能力。此菌株能在宿主植物體內定殖，並已證實可促進香蕉、阿拉伯芥及多種葉菜類作物的生長。我們進一步顯示，在正常、鹽分及乾旱逆境條件下，接種 B. seminalis 869T2 的阿拉伯芥與小白菜，其多項生長指標皆顯著優於未接種對照組。轉錄體分析與即時定量 PCR 結果亦指出，在鹽分逆境下接種 869T2 的阿拉伯芥，其與植物激素訊息傳遞相關之基因（包含植物生長素、吉貝素、細胞分裂素與離素）的表現量，與鹽處理對照組相比皆有明顯改變。此外，在鹽分與乾旱逆境下，接種 869T2 菌株的阿拉伯芥與小白菜，其過氧化氫（H₂O₂）累積量、電解質滲漏（EL）與丙二醛（MDA）含量均低於對照組。綜合而言，B. seminalis 869T2 這株植物內生菌可透過影響多種植物激素反應並降低氧化壓力傷害，進而提升宿主植物對鹽分與乾旱逆境的耐受性。 |
| 英文摘要 | Due to global warming and gradual climate change, plants are subjected to a wide range of environmental stresses, adversely affecting plant growth and production worldwide. Plants have developed various mechanisms to overpower these abiotic stresses, including salt stress, drought, and high light intensity. Apart from their own defense strategies, plants can get help from the beneficial endophytic bacteria inside host plants and assist them in enduring severe growth conditions. A previously isolated plant endophytic bacteria, Burkholderia seminalis 869T2, from vetiver grass can produce auxin, synthesize siderophore, and solubilize phosphate. The B. seminalis 869T2 can colonize inside host plants and increase the growth of bananas, Arabidopsis, and several leafy vegetables. We further demonstrated that different growth parameters of Arabidopsis and pak choi plants were significantly increased after inoculating the B. seminalis 869T2 under normal, salt, and drought stress conditions compared to the mock-inoculated plants. Both transcriptome analysis and quantitative real-time PCR results showed that expression levels of genes related to phytohormone signal transduction pathways, including auxin, gibberellin, cytokinin, and abscisic acid were altered in Arabidopsis plants after inoculated with the strain 869T2 under salt stress, in comparison to the mock-inoculated control with salt treatments. Furthermore, the accumulation levels of hydrogen peroxide (H2O2), electrolyte leakage (EL), and malondialdehyde (MDA) were lower in the 869T2-inoculated Arabidopsis and pak choi plants than in control plants under salt and drought stresses. The plant endophytic bacterium strain B. seminalis 869T2 may affect various phytohormone responses and reduce oxidative stress damage to increase salt and drought stress tolerances of host plants. |
| 發表成果與本中心研究主題相關性 | 施用植物內生菌可幫助作物在高鹽或乾旱逆境下提升生長速率，相較於化學肥料，可作為高效能且對環境友善的替代方案。 |
