【學術亮點】A Microfluidic Platform Integrating Dielectrophoretic Concentration and Impedimetric Sensing for Rapid Salmonella choleraesuis Detection
Facility Agricultural: Utilization of Livestock Waste in Automated Cultivation of Microalgae and Reutilization in Poultry Farming【Department of Bio-industrial Mechatronics Engineering / Wu, Ching-Chou / Distinguished Professor】
設施農業：畜牧廢棄物於小球藻自動栽培與禽類再利用【生物產業機電工程學系/吳靖宙特聘教授】
| 論文篇名 | 英文：A Microfluidic Platform Integrating Dielectrophoretic Concentration and Impedimetric Sensing for Rapid Salmonella choleraesuis Detection 中文：整合介電泳濃縮與阻抗式感測之微流體晶片用於快速偵測 Salmonella choleraesuis |
| 期刊名稱 | ACS Sensors |
| 發表年份,卷數,起迄頁數 | 2025, 10(9), 6512–6520 |
| 作者 | Patil, Avinash V. Police; Chuang, Yu-Sheng; Lin, Che-Wei; Yang, Chiou-Ying; Yasukawa, Tomoyuki; Wu, Ching-Chou(吳靖宙)* |
| DOI | 10.1021/acssensors.5c00779 |
| 中文摘要 | 對於食品安全與公共衛生管控，快速且高靈敏地檢測食源性細菌（如沙門氏菌）至關重要。本研究建立一款微流體裝置，整合上下對置（top–bottom）電極對，用於執行正介電泳（pDEP）與電化學阻抗頻譜（EIS）。以抗沙門氏菌抗體修飾之免疫磁珠（IMBs）加入含有腸沙門氏菌 Salmonella enterica Choleraesuis 血清型（SC）的樣品中，孵育 30 分鐘形成 IMBs@SC 複合物，並以磁力分離，以避免樣品基質汙染 EIS 偵測電極。接著在工作電極（WE）的微孔結構上施加 50 kHz／7.5 Vpp 的 pDEP 3 分鐘以收集 IMBs@SC 複合物，之後以 EIS 判定 SC 濃度。於未稀釋牛奶中摻入 SC 的測試顯示，線性範圍為 101–104 CFU/mL，推算偵測極限為 2 CFU/mL。從 IMBs 免疫反應到裝置上取得結果的整體流程僅需 50 分鐘。此微流體裝置為快速且超高靈敏之致病菌檢測提供了具前景的平台。 |
| 英文摘要 | Fast and sensitive detection of foodborne bacteria, such as Salmonella, is essential for food safety and public health control. This study constructed a microfluidic device integrating a top-bottom opposite electrode pair for performing positive dielectrophoresis (pDEP) and electrochemical impedance spectroscopy (EIS). The immunomagnetic beads (IMBs) modified with anti-Salmonella antibodies were spiked in Salmonella enterica serovar Choleraesuis (SC)-containing samples for 30 min to form IMBs@SC complexes, and they were separated by a magnet, which can prevent the sample matrix from contaminating the EIS detectors. After applying the 50 kHz/7.5 Vpp pDEP for 3 min to collect the IMBs@SC complexes in the microholes of the working electrodes, EIS was used to determine the SC concentration. The linear range and the calculated detection limit obtained from SC-spiked undiluted milk samples are 101 to 104 and 2 cfu/mL. The total procedures from the IMBs immunoreaction to the sample-to-result on the device only take 50 min. The microfluidic device offers a promising platform for the rapid and ultrasensitive detection of pathogenic bacteria. |
| 發表成果與本中心研究主題相關性 | 對於食品安全與公共衛生管控，能快速且高靈敏地檢測食源性細菌（如沙門氏菌）至關重要。本研究建立一款微流體裝置，以正介電泳與電化學阻抗頻譜能在50 分鐘內獲得結果，此裝置為快速且超高靈敏之致病菌檢測提供了具前景的平台。 |
