【學術亮點】Exploring the interaction dynamics of growth-promoting bacterial endophytes and fertilizer on Oryza sativa L. under heat stress
Recycling Agriculture: Development of Integrated All-in-One Technology for the Innovative carbon sequestration of Organic Circular Agriculture【Department of Soil Environmental Sciences / Shen, Fo-Ting / Professor】
循環農業：農業有機循環再利用的多合一創新碳匯技術開發【土壤環境科學系/沈佛亭教授】
| 論文篇名 | 英文：Exploring the interaction dynamics of growth-promoting bacterial endophytes and fertilizer on Oryza sativa L. under heat stress 中文：探討熱逆境下具促進植物生長之內生細菌與肥料對於水稻生長之影響 |
| 期刊名稱 | Rice |
| 發表年份,卷數,起迄頁數 | 2025, 18, no.33 |
| 作者 | Dlamini, Wonder Nathi; Yu, Kuo-Pin; Chen, Wen-Ching; Shen, Fo-Ting(沈佛亭)* |
| DOI | 10.1186/s12284-025-00781-9 |
| 中文摘要 | 水稻（Oryza sativa L.）作為主要糧食作物的需求持續增加，但氣候變遷導致的氣溫上升對其生產構成嚴重威脅。本研究旨在利用內生細菌與肥料的結合，以減輕高溫對水稻生長的不利影響。研究中將接種與未接種內生菌的幼苗，在常溫與40–45°C熱逆境條件下進行生長參數比較。分離出的菌株經過DNA萃取與16S rRNA基因定序鑑定，並分析其促進植物生長之特性。在受控環境中，將水稻幼苗以25°C栽培14天後，再以40°C處理持續10天，並觀察肥料與耐熱內生菌對水稻幼苗生長的影響。另進行為期150天的盆栽試驗，將植物置於35°C下生長60天，再迅速升溫至40°C持續30天，最後降回35°C再栽培60天。接種內生菌後，水稻的穗部發育明顯改善，植株生物量與株高皆增加；其中接種菌株W（Bacillus paralicheniformis）時，新鮮穀粒重量提升達50%。相比對照組，菌株W、N（B. pumilus）與 D（B. paranthracis）分別使每穗乾穀重量增加 113%、83% 與 87%。在熱逆境條件下，菌株W表現出最顯著的增產效果。經150天熱逆境並施用半量肥料之處理，植株丙二醛（MDA）含量降低。接種內生菌能提高脯氨酸（proline）、水楊酸鹽（salicylate）與離層酸（abscisic acid）的含量，顯示其可減輕滲透逆境效應。此結果突顯內生細菌在誘導水稻細胞內生理活性反應中的重要作用。尤其菌株W、N、D與 NPK50肥料結合使用時，展現出顯著促進植物生長並緩解高溫逆境的潛力。 |
| 英文摘要 | The demand for rice (Oryza sativa L.) as a staple food continues to grow, but rising temperatures due to climate change pose a significant threat to its production. This study addresses the challenge by employing endophytic bacteria and fertilizer to mitigate the adverse effects of high temperatures on rice plants. Seedlings were evaluated for growth parameters, comparing outcomes with non-inoculated counterparts under normal and 40 to 45 °C heat shock conditions. Isolates underwent thorough DNA extraction and 16 S rRNA gene sequencing for identification and were scrutinized for their plant growth-promoting (PGP) traits. The effects of fertilizer and thermotolerant bacteria on rice plants were investigated in controlled chambers at 25 °C for 14 days, succeeded by exposure to 40 °C for 10 days. A consecutive soil pot experiment extended over 150 days, exposing plants to growth chambers set at 35 °C for 60 days, followed by a rapid increase to 40 °C for 30 days and a subsequent reduction to 35 °C for an additional 60 days. Inoculating with the isolates resulted in panicle development and increased plant biomass and length, with fresh grain weights showing a 50% improvement when using bacterial strain W (B. paralicheniformis). Additionally, dry grain weights per panicle rose by 113% with strain W, 83% with strain N (B. pumilus), and 87% with strain D (B. paranthracis) compared to the control. Bacterial strain W exhibited the most pronounced effect on rice yield under heat stress. The results demonstrated a decrease in malondialdehyde (MDA) levels after 150 days of heat stress and half-dose of the recommended fertilizer. Bacterial inoculation increased proline, salicylate, and abscisic acid content, suggesting the alleviation of osmotic stress effects. This highlights the role of endophytic bacteria in stimulating biologically active responses within rice plant cells. Notably, bacterial strains W, N, and D show potential for enhancing plant growth and mitigating heat stress when used in conjunction with NPK50. |
| 發表成果與本中心研究主題相關性 | 本研究指出具促進植物生長的內生菌結合肥料可提升水稻在高溫下的耐受性與產量，減少化肥依賴與環境負擔。透過促進植物抗氧化與滲透調節機制，上述微生物可在氣候變遷下維持作物生產穩定。此技術屬於永續農業創新，兼顧糧食安全與生態保育，推動低投入、高效率、環境友善的農業發展方向。 |
