Recycling Agriculture: Development of Integrated All-in-One Technology for the Innovative carbon sequestration of Organic Circular Agriculture【Department of Soil Environmental Sciences / Young, Chiu-Chung / Academician】
循環農業：農業有機循環再利用的多合一創新碳匯技術開發【土壤環境科學系/楊秋忠院士】
論文篇名 英文：Description of Flavobacterium agricola sp. nov., an auxin producing bacterium isolated from paddy field
中文：一株可合成auxin生長素之台灣本土新穎農業黃桿菌之描述
期刊名稱 ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
發表年份,卷數,起迄頁數 2023; 116, 1345-1357
作者 Lin, Shih-Yao; Hameed, Asif; Tsai, Chia-Fang; Young, Chiu-Chung(楊秋忠)*
DOI 10.1007/s10482-023-01891-4
英文摘要 An auxin-producing bacterial strain, CC-SYL302T, was isolated from paddy soil in Taiwan and identified using a polyphasic taxonomic approach. The cells were observed to be aerobic, non-motile, non-spore-forming rods, and tested positive for catalase and oxidase. Produced carotenoid but flexirubin-type pigments were absent. Optimal growth of strain CC-SYL302T was observed at 25 °C, pH 7.0, and with 2 % (w/v) NaCl present. Based on analysis of 16S rRNA gene sequences, it was determined that strain CC-SYL302T belongs to the genus Flavobacterium of the Flavobacteriaceae family. The closest known relatives of this strain are F. tangerinum YIM 102701-2T (with 93.3 % similarity) and F. cucumis R2A45-3T (with 93.1 % similarity). Digital DNA-DNA hybridization (dDDH) values were calculated to assess the genetic distance between strain CC-SYL302T and its closest relatives, with mean values of 21.3 % for F. tangerinum and 20.4 % for F. cucumis. Strain CC-SYL302T exhibited the highest orthologous average nucleotide identity (OrthoANI) values with members of the Flavobacterium genus, ranging from 67.2 % to 72.1 % (n=22). The dominating cellular fatty acids (>5 %) included iso-C14:0, iso-C15:0, iso-C16:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C16:1 ω6c / C16:1 ω7c and C16:0 10-methyl / iso-C17:1 ω9c. The polar lipid profile consisted of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and nine unidentified polar lipids. The genome (2.7 Mb) contained 33.6 % GC content, and the major polyamines were putrescine and sym-homospermidine. Strain CC-SYL302T exhibits distinct phylogenetic, phenotypic, and chemotaxonomic characteristics, as well as unique results in comparative analysis of 16S rRNA gene sequence, OrthoANI, dDDH, and phylogenomic placement. Therefore, it is proposed that this strain represents a new species of the Flavobacterium genus, for which the name Flavobacterium agricola sp. nov. is proposed. The type strain is CC-SYL302T (=BCRC 81320T= JCM 34764T).
發表成果與本中心研究主題相關性 本研究的目的是描述一株從台灣的稻田土壤中分離出可合成auxin生長素之台灣本土新穎農業黃桿菌菌株 CC-SYL302T，並使用多相分類方法進行鑑定。計算數字 DNA-DNA 雜交 (dDDH) 值以評估菌株 CC-SYL302T 與其最近親之間的遺傳距離，F. tangerinum 的平均值為 21.3 %，F. cucumis 的平均值為 20.4 %。 菌株 CC-SYL302T 與黃桿菌屬成員表現出最高的直系同源平均核苷酸同一性 (OrthoANI) 值，範圍為 67.2% 至 72.1% (n=22)。 主要細胞脂肪酸 (>5%) 包括 iso-C14:0、iso-C15:0、iso-C16:0、iso-C15:0 3-OH、iso-C17:0 3-OH、C16:1 ω6c / C16:1 ω7c 和C16:0 10-甲基/異-C17:1 ω9c。基因組 (2.7 Mb) 含有 33.6% GC 含量，主要多胺是腐胺和 sym-homospermidine。CC-SYL302T菌株表現出獨特的系統發育、表型和化學分類學特徵，以及在16S rRNA基因序列、OrthoANI、dDDH和系統發育位置的比較分析中獨特的結果。因此確認該菌株代表了黃桿菌屬的一個新種，其名稱為農業黃桿菌。