設施農業：智慧感測【生物產業機電工程學系/吳靖宙教授】
| 論文篇名 | 英文：Dissolved Oxygen-Sensing Chip Integrating an Open Container Connected with a Position-Raised Channel for Estimation of Cellular Mitochondrial Activity 中文：整合位置舉離通道之開放微槽的溶氧感測晶片以評估細胞粒線體活性評估 |
| 期刊名稱 | ACS SENSORS |
| 發表年份,卷數,起迄頁數 | 2022, 7, 7, 1808–1818. |
| 作者 | Wu, Ching-Chou(吳靖宙)*; Wang, Chieh-Jen; Chang, Cicero Lee-Tian; Shiku, Hitoshi; Wang, Yu-Ren; Yan, Jia-De; Ding, Shinn-Jyh |
| DOI | 10.1021/acssensors.1c02287 |
| 中文摘要 | 對貼附型細胞的氧氣消耗率量測在細胞生物能量健康與代謝活性的評估是一重要議題。本研究建構一整合位置舉離通道之開放微槽的溶氧(DO)感測晶片，藉由黃金超微電極可量化貼附於微槽內細胞的氧氣消耗率(OCR)。該位置舉離通道的設計可降低流體更換時的剪切力，以避免影響細胞與電極間的相對位置。該超微電極在40-60 s的量測區間具有極佳的再現性(1.1%相對標準差)，經90 min培養吸附後，即可經時性量化3T3-L1細胞受到外界藥物刺激的OCR變化，藉由粒線體抑制藥物的添加，可了解細胞粒線體的健康程度。 |
| 英文摘要 | The measurement of oxygen consumption of adherent cells is a profoundly important issue for estimating the bioenergetic health and metabolism activity of cells. The study describes the construction of a microfluidic chip consisting of an open container connected with a position-raised channel and dissolved oxygen (DO)-sensing gold ultramicroelectrodes for quantifying the oxygen consumption rate (OCR) of adherent cells. The microfluidic chip design can reduce the action of shear force on the adherent cells during medium replacement. The residual concentration of analytes in the open container was only 4.4% after solution replacement via the position-raised channel. The DO reduction current measured by ultramicroelectrodes averaged in the range of 40-60 s presented high reproducibility with a 1.1% relative standard deviation suitable for OCR calculation. After short-term (90 min) cultivation, the microfluidic chip can monitor the time-dependent change in the OCR of 3T3-L1 cells for several hours by repeatedly replacing the culture medium or with the stimulation of different mitochondrial inhibitors. The presented microfluidic cell-based chip has great promise for drug screening and chemosensitivity testing by measuring OCR to evaluate the mitochondrial function of adherent cells |
| 發表成果與本中心研究主題相關性 | 使用整合位置舉離通道之開放微槽的溶氧感測晶片可在短時間內進行細胞粒線體活性的評估，可用於環境用藥、奈米粒子或病原菌等對動物細胞或植物細胞的生理活性經時性影響評估。 |
