【學術亮點】Pantoea piersonii IIIF1SW-P2T triggers cytotoxicity through L-asparaginase-driven ammonium secretion and colonizes reproductive tract of Caenorhabditis elegans
Recycling Agriculture: Development of Integrated All-in-One Technology for the Innovative carbon sequestration of Organic Circular Agriculture【Department of Soil Environmental Sciences / Young, Chiu-Chung / Academician】
循環農業：農業有機循環再利用的多合一創新碳匯技術開發【土壤環境科學系/楊秋忠院士】
| 論文篇名 | 英文：Pantoea piersonii IIIF1SW-P2T triggers cytotoxicity through L-asparaginase-driven ammonium secretion and colonizes reproductive tract of Caenorhabditis elegans 中文： Pantoea piersonii IIIF1SW-P2T 透過 L-天門冬醯胺酶介導的銨離子分泌誘發細胞毒性並於秀麗隱桿線蟲生殖道中定殖 |
| 期刊名稱 | Scientific Reports |
| 發表年份,卷數,起迄頁數 | 2025, 15, no.35295 |
| 作者 | Hameed, Asif; Young, Chiu-Chung(楊秋忠)*; Suchithra, Kokkarambath Vannadil; Prabhu, Ashwini; Dhanyashree, Honagodu Ravichandra; Shastry, Rajesh Padumane |
| DOI | 10.1038/s41598-025-19368-x |
| 中文摘要 | 本研究探討來自國際太空站的細菌株 Pantoea piersonii IIIF1SW-P2T。原先預測其無致病性，但研究顯示其重組酶 L-asparaginase（Kp_AnsA）能將 L-asparagine 分解為天冬氨酸並產生氨離子（NH₄⁺），對人類胚胎腎細胞（HEK 293T）造成顯著細胞毒性。該酶在鹼性條件（最佳 pH 9）下活性最高，並具耐鹽性，與海洋細菌的同類酶具有高度序列相似性。實驗進一步發現，帶有綠色螢光標記的 IIIF1SW-P2T 可迅速且穩定地定殖於秀麗隱桿線蟲（C. elegans）的生殖道，主要集中於外陰及子宮區域，並造成輕微死亡（約 5%）。結果顯示，該菌的 L-asparaginase 透過氨生成引發細胞毒性，並具備在真核宿主生殖組織定殖的潛能，提供研究微生物與宿主生殖健康交互作用的新模型。 |
| 英文摘要 | The study investigates Pantoea piersonii IIIF1SW-P2T, a bacterial strain isolated from the International Space Station, previously thought to be non-pathogenic. Researchers discovered that its recombinant L-asparaginase enzyme (Kp_AnsA) converts L-asparagine into aspartic acid and releases ammonium (NH₄⁺), which causes significant cytotoxicity in human HEK 293T cells. The enzyme shows high activity at alkaline pH (optimum pH 9) and tolerance to various metal ions, sharing sequence identity with marine bacterial asparaginases. In Caenorhabditis elegans, GFP-tagged IIIF1SW-P2T rapidly and stably colonized the reproductive tract, particularly at the vulva and uterine regions, with mild host mortality (~5%). This work demonstrates that NH₄⁺ generation by bacterial L-asparaginase contributes to cytotoxicity and reveals the bacterium’s potential to colonize eukaryotic reproductive tissues, suggesting a possible model for studying host-microbe interactions affecting reproductive health. |
| 發表成果與本中心研究主題相關性 | 本研究揭示微生物的氨生成與胺基酸代謝機制對農業永續有潛在價值，有助於開發生物基氮管理技術，以取代化學肥料、降低氨排放與碳足跡。該菌種的耐鹽與太空環境適應特性，可提供極端環境微生物在土壤復育、鹽害治理與循環生物肥料開發上的應用潛力，其對微生物代謝、環境適應與氮循環利用可為未來節能減碳與生態平衡導向的永續農業提供科學基礎。 |
