新穎材料農業：友善環境農業新穎材料研發與安全評估【材料科學與工程學系/薛涵宇副教授】
| 論文篇名 | 英文：Hydrodynamic Snaring Array for Trapping and Perfusion Culture of Single Cell 中文：流體動力學圈套陣列用於捕獲和灌注培養單株細胞 |
| 期刊名稱 | Sensors and Actuators B: Chemical |
| 發表年份,卷數,起迄頁數 | 2020, 312, no.127966 |
| 作者 | Chiang, Ya-Yu; Teng, Yi-Chiuan*; Su, Zhe-Yi; Hsueh, Han-Yu(薛涵宇); Tu, Kai-Hung |
| DOI | 10.1016/j.snb.2020.127966 |
| 中文摘要 | 本研究開發了流體動力學圈套器與微孔陣列以完成單細胞捕獲和幹細胞灌注。本研究開發獨特的 V 形器具加載單一骨髓幹細胞，進一步誘導定向流線分佈形成 U 形流場。此器具內含之空隙降低了細胞流域內的流速並實現了穩定的灌注，流體動力學圈套陣列與線上同步溫度控制和監測相結合，可消除紫外線對顯微鏡的照射影響，適合長時間使用，降低外部干預，進而促進單一骨髓幹細胞的生長。 |
| 英文摘要 | A hydrodynamic snare and well array were developed to accomplish single-cell trapping and stem-cell perfusion culture. Directed streamline distribution is induced using unique V-shaped weir to load single bone marrow stem cell into U-shaped dwelling weir. The voids decrease flow speed in the dwelling area and enable a stable perfusion culture. The hydrodynamic snaring array was integrated with an on-line temperature control and monitoring system, and the ultraviolet illumination effect on a microscope is eliminated that is suitable for the long-term growth of single bone marrow stem cell with minimal external interventions. |
| 發表成果與本中心研究主題相關性 | 此研究開發一新穎製程, 著重於幹細胞捕獲與培養, 此技術可提供給畜產相關領域, 有助於畜牧業培育幹細胞之跨領域研究。 |
