論文篇名 英文：Metabolic potential and community structure of bacteria in an organic tea plantation
中文：有機茶園土壤中細菌代謝潛勢與群落結構
期刊名稱 APPLIED SOIL ECOLOGY
發表年份,卷數,起迄頁數 2021, 157, 103762
作者 Chen, Wen-Ching; Ko, Chi-Hsuan; Su, Yen-Shuo; Lai, Wei-An; Shen, Fo-Ting(沈佛亭)*
DOI 10.1016/j.apsoil.2020.103762
中文摘要 為探討有機茶園土壤細菌之時空分布特性，本研究利用群落層次生理圖譜分析與16S rDNA次世代定序，解析一年四季有機茶園土壤、茶樹根面與茶樹根內之細菌群落結構。研究結果顯示，有機質肥料施用後會造成土壤pH值、EC值與有機質含量發生短期的擾動。由PCA、PCoA和UPGMA分析結果可得知茶園土壤、茶樹根面與茶樹根內之細菌群落組成明顯不同，其中根面細菌群落有較高的碳代謝潛勢，茶樹根內細菌群落則呈現較低的碳代謝潛勢。雖然細菌群落多樣性與其結構在不同季節呈現變動趨勢，但仍可發現相較於根內細菌群落，土壤細菌群落與根面細菌群落有較高的碳代謝潛勢與多樣性。在土壤與根面細菌群落分析中可發現Gemmataceae、Pedosphaeraceae與Solibacteraceae為主要存在的優勢菌科，Acidothermaceae、Burkholderiaceae、Ktedonobacteraceae與Xanthobacteraceae則為根內細菌群落中的優勢菌科。Burkholderiaceae與 Xanthobacteraceae兩菌科無論在茶樹根面或根內均具有較高的相對豐富度，值得進一步探討其與茶樹生長之相關性。
英文摘要 Revealing the spatial and temporal distribution of bacteria in organic tea plantations is of great importance to clarify soil- and/or tea plant-associated community. In this study samples were collected from three rhizo-compartments of tea plant during four seasons, which were further subjected to community level physiological profiling and 16S rDNA next generation sequencing. Organic fertilization caused a short-term disturbance on pH, EC, and organic matter content of soil. Three rhizo-compartments harbored distinct bacterial community as revealed by PCA, PCoA and UPGMA analysis. Utilization of six carbon source categories was positively correlated with root surface community while negatively correlated with root interior community. Although there were variations in microbial parameters during four seasons, higher metabolic potential and diversity were observed in soil-associated rhizo-compartments. Bacterial families Gemmataceae, Pedosphaeraceae, and Solibacteraceae were more abundant in soil and root surface, while root interior were dominated by families Acidothermaceae, Burkholderiaceae, Ktedonobacteraceae, and Xanthobacteraceae. It is proposed that Burkholderiaceae and Xanthobacteraceae which dominate in root surface or root interior samples and other poorly studied phylogenetic groups may serve as potential candidates to study their interaction with tea plant.
發表成果與本中心研究主題相關性 本研究旨在探討有機茶園土壤細菌之時空分布特性，並解析不同季節有機茶園土壤、茶樹根面與茶樹根內之細菌群落結構，研究成果有助於茶園土壤之健康管理與永續發展。