設施農業：畜牧廢棄物於小球藻自動栽培與禽類再利用【生物產業機電工程學系/吳靖宙教授】
| 論文篇名 | 英文：Rapid detection of mango allergen in processed foods using an immunomagnetic nanoparticle-based electrochemical immunosensor 中文：使用基於免疫磁性奈米顆粒的電化學免疫感測器快速檢測加工食品中的芒果過敏原 |
| 期刊名稱 | Microchemical Journal |
| 發表年份,卷數,起迄頁數 | 2024, 198, 110070 |
| 作者 | Wu, Ching-Chou(吳靖宙); Lin, Chia -Chen; Yin, Hsin-Yi; Tsai, Wen-Che; Yang, Ping-Feng; Liu, Hung-Jen; Wen, Hsiao-Wei |
| DOI | 10.1016/j.microc.2024.110070 |
| 中文摘要 | 本研究介紹了一種基於免疫磁性奈米顆粒（IMNP）的安培免疫感測器，用於檢測40-kDa主要芒果過敏原，這些過敏原已知可能引起敏感性個體的致命過敏性休克。通過利用IMNP，該檢測方法選擇性地從食品樣品中分離和濃縮40-kDa主要芒果過敏原，然後使用固定在金奈米顆粒（AuNP）修飾的網版印刷碳電極上的單株抗體進行特異性捕捉。利用HQ/H2O2系統透過過氧化物酵素偶聯的二抗體實現檢測。在優化的條件下，免疫感測器表現出卓越的特異性，可有效區分芒果與其他九種水果和蔬菜，並實現40-kDa 主要芒果過敏原的低檢測限(LOD)，緩衝液中為1.45 μg/ mL，果汁中為2.25 μg/mL ，乳酸飲料中 1.45 μg/mL。使用 24 種商業食品對測定進行驗證，並透過蛋白質印跡順序分析這些食品以確認免疫感測器的結果。此驗證證明了該檢測具有 100% 的出色特異性和 91.7% 的高靈敏度。這些結果強調了免疫感測器能夠快速、靈敏地檢測加工食品中 40-kDa 主要芒果過敏原，從而顯著改進過敏原檢測技術。 |
| 英文摘要 | This work presents an immunomagnetic nanoparticle (IMNP)-based amperometric immunosensor designed for the detection of 40-kDa major mango allergens, known to potentially induce fatal anaphylactic shock in susceptible individuals. By utilizing IMNPs, the assay selectively isolates and concentrates 40-kDa major mango allergens from food samples, followed by their specific capture using monoclonal antibodies immobilized on gold nanoparticles (AuNPs)-modified screen-printed carbon electrodes. Detection is achieved through a peroxidase-conjugated secondary antibody utilizing an HQ/H2O2 system. With optimized conditions, the immunosensor exhibits exceptional specificity, effectively distinguishing mango from nine other fruits and vegetables, and achieving low limits of detection (LOD) for 40-kDa major mango allergens at 1.45 μg/mL in buffer, 2.25 μg/mL in juice, and 1.45 μg/mL in lactic acid beverage. Validation of the assay was performed using 24 commercial food products, which were analyzed sequentially by Western blot to confirm the immunosensor's results. This validation demonstrated the assay's outstanding specificity at 100 % and high sensitivity at 91.7 %. These results emphasize the immunosensor's capability for rapid and sensitive detection of 40-kDa major mango allergens in processed foods, offering a significant improvement in allergen detection technology. |
| 發表成果與本中心研究主題相關性 | 本研究開發了一種安培法免疫感測器，用於檢測40-kDa芒果過敏原。金奈米粒子和免疫奈米磁珠能有效地放大檢測訊號，且具有良好的檢測極限與良好的選別性，能用於加工芒果產品或是其他加工農產品的過敏原快速檢測。 |
